Lipases as Tools in the Synthesis of Prodrugs from Racemic 9-(2,3-Dihydroxypropyl)adenine

Lipases from Geotrichum candidum 4013 (extracellular lipase and cell-bound lipase) were immobilized by adsorption on chitosan beads. The enzyme preparations were tested in the synthesis of ester prodrugs from racemic 9-(2,3-dihydroxypropyl)adenine in dimethylformamide with different vinyl esters (ac...

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Bibliographic Details
Published in:Molecules
Main Authors: Brabcová, J. (Jana), Blažek, J. (Jiří), Janská, L. (Lucie), Krečmerová, M. (Marcela), Zarevúcka, M. (Marie)
Format: Article in Journal/Newspaper
Language:English
Published: 2012
Subjects:
lipase
transesterification
prodrug
immobilization of enzymes
Antarc*
Antarctica
Online Access:https://doi.org/10.3390/molecules171213813
http://hdl.handle.net/11104/0214185
Description
Summary:Lipases from Geotrichum candidum 4013 (extracellular lipase and cell-bound lipase) were immobilized by adsorption on chitosan beads. The enzyme preparations were tested in the synthesis of ester prodrugs from racemic 9-(2,3-dihydroxypropyl)adenine in dimethylformamide with different vinyl esters (acetate, butyrate, decanoate, laurate, palmitate). The transesterification activities of these immobilized enzymes were compared with commercially available lipases (lipase from hog pancreas, Aspergillus niger, Candida antarctica, Pseudomonas fluorescens).

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