Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard

The arctic freshwater bacterium Pseudomonas fluorescens BD5 produces biosurfactants when grown on 2 percent glucose. Crude biosurfactants were extracted from a cell-free culture supernatant with ethyl acetate and purified by preparative reversed phase high performance liquid chromatography (RP-HPLC)...

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Bibliographic Details
Published in:Bioresource Technology
Main Authors: Janek, T., Lukaszewicz, M., Řezanka, T. (Tomáš), Krasowska, A.
Format: Article in Journal/Newspaper
Language:English
Published: 2010
Subjects:
Pseudomonas fluorescens BD5
Lipopeptide
Pseudofactins
Arctic Archipelago
Svalbard
Online Access:https://doi.org/10.1016/j.biortech.2010.02.109
http://hdl.handle.net/11104/0190858
Description
Summary:The arctic freshwater bacterium Pseudomonas fluorescens BD5 produces biosurfactants when grown on 2 percent glucose. Crude biosurfactants were extracted from a cell-free culture supernatant with ethyl acetate and purified by preparative reversed phase high performance liquid chromatography (RP-HPLC). The chemical structure of the purified biosurfactants, pseudofactin I and II, was analyzed by matrix assisted laser desorption/ionization time of flight (MALDI TOF) mass spectrometry and tandem mass spectrometry (MS/MS). Both compounds are novel cyclic lipopeptides with a palmitic acid connected to the terminal amino group of eighth amino acid in peptide moiety

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