Cytotoxic activity against prelabelled RTG-2 cells in the turbot, Scophthalmus maximus (L.)

• Published in: Journal of Fish Diseases
• Main Authors: Tafalla, Carolina, Figueras Huerta, Antonio, Novoa, Beatriz
• Format: Article in Journal/Newspaper
• Language: English
• Published: Blackwell Publishing 2001
• Subjects: Turbot; Leucocytes; Cytotoxicity; Fish; Non-specific natural cytotoxic cells; Immune system; Scophthalmus maximus
• Online Access: http://hdl.handle.net/10261/56858; https://doi.org/10.1046/j.1365-2761.2001.00288.x

7 páginas, 5 figuras Leucocytes derived from head kidney, blood and spleen of turbot, Scophthalmus maximus (L.), exhibited cytotoxic activities against an established cell line (RTG-2) derived from rainbow trout, Oncorhynchus mykiss (Walbaum). The optimal effector:target ratio, temperature and incubation period for the activity was determined. Cytotoxicity was not caused by released factors, as effector:target contact was needed. Visualization of the cultures under the transmission electron microscope corroborated the contact between leucocytes and target cells and suggested the implication of monocyte-like cells in this cell-mediated cytotoxicity. This research was supported by grant MAR 96-1775 from the ComisioÂn Interministerial de Ciencia y TecnologõÂa (CICYT). Peer reviewed