Cytotoxic activity against prelabelled RTG-2 cells in the turbot, Scophthalmus maximus (L.)

7 páginas, 5 figuras Leucocytes derived from head kidney, blood and spleen of turbot, Scophthalmus maximus (L.), exhibited cytotoxic activities against an established cell line (RTG-2) derived from rainbow trout, Oncorhynchus mykiss (Walbaum). The optimal effector:target ratio, temperature and incub...

Full description

Bibliographic Details
Published in:Journal of Fish Diseases
Main Authors: Tafalla, Carolina, Figueras Huerta, Antonio, Novoa, Beatriz
Format: Article in Journal/Newspaper
Language:English
Published: Blackwell Publishing 2001
Subjects:
Turbot
Leucocytes
Cytotoxicity
Fish
Non-specific natural cytotoxic cells
Immune system
Scophthalmus maximus
Online Access:http://hdl.handle.net/10261/56858
https://doi.org/10.1046/j.1365-2761.2001.00288.x
Description
Summary:7 páginas, 5 figuras Leucocytes derived from head kidney, blood and spleen of turbot, Scophthalmus maximus (L.), exhibited cytotoxic activities against an established cell line (RTG-2) derived from rainbow trout, Oncorhynchus mykiss (Walbaum). The optimal effector:target ratio, temperature and incubation period for the activity was determined. Cytotoxicity was not caused by released factors, as effector:target contact was needed. Visualization of the cultures under the transmission electron microscope corroborated the contact between leucocytes and target cells and suggested the implication of monocyte-like cells in this cell-mediated cytotoxicity. This research was supported by grant MAR 96-1775 from the ComisioÂn Interministerial de Ciencia y TecnologõÂa (CICYT). Peer reviewed

Similar Items