Cytotoxic activity against prelabelled RTG-2 cells in the turbot, Scophthalmus maximus (L.)
7 páginas, 5 figuras Leucocytes derived from head kidney, blood and spleen of turbot, Scophthalmus maximus (L.), exhibited cytotoxic activities against an established cell line (RTG-2) derived from rainbow trout, Oncorhynchus mykiss (Walbaum). The optimal effector:target ratio, temperature and incub...
Published in: | Journal of Fish Diseases |
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Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Blackwell Publishing
2001
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Subjects: | |
Online Access: | http://hdl.handle.net/10261/56858 https://doi.org/10.1046/j.1365-2761.2001.00288.x |
Summary: | 7 páginas, 5 figuras Leucocytes derived from head kidney, blood and spleen of turbot, Scophthalmus maximus (L.), exhibited cytotoxic activities against an established cell line (RTG-2) derived from rainbow trout, Oncorhynchus mykiss (Walbaum). The optimal effector:target ratio, temperature and incubation period for the activity was determined. Cytotoxicity was not caused by released factors, as effector:target contact was needed. Visualization of the cultures under the transmission electron microscope corroborated the contact between leucocytes and target cells and suggested the implication of monocyte-like cells in this cell-mediated cytotoxicity. This research was supported by grant MAR 96-1775 from the ComisioÂn Interministerial de Ciencia y TecnologõÂa (CICYT). Peer reviewed |
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