Differentiation of raw or processed eel by PCR-based techniques: Restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP)
7 páginas, 7 figuras, 3 tablas.-- Hartmut Rehbein . et al. PCR-based methods have been developed to differentiate four species of commercially important eels (Anguilla anguilla, A. rostrata, A. japonica, A. australis). By means of universal primers a 464 base pair (bp) section of the cytochrome b ge...
| Published in: | European Food Research and Technology |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Springer
2002
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10261/56478 https://doi.org/10.1007/s00217-001-0457-y |
| Summary: | 7 páginas, 7 figuras, 3 tablas.-- Hartmut Rehbein . et al. PCR-based methods have been developed to differentiate four species of commercially important eels (Anguilla anguilla, A. rostrata, A. japonica, A. australis). By means of universal primers a 464 base pair (bp) section of the cytochrome b gene was amplified. The amplicon was sequenced and restriction enzymes were selected for RFLP analysis; a short part (123 bp) of the 464 bp section was amplified by another set of primers and used for SSCP analysis. The suitability of RFLP and SSCP for differentiating hot-smoked eel was verified by a collaborative study The authors wish to thank the European Commission for the financial support for this study (FAIR CT973061). Peer reviewed |
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