Isolation of a highly pathogenic Vibrio pelagius strain associated with mass mortalities of turbot, Scophthalmus maximus (L.), larvae

11 páginas, 7 figuras, 2 tablas A bacterial strain, characterized as Vibrio pelagius (Hq 222), was isolated from a turbot, Scophthalmus maximus (L.), larvae mass mortality in a commercial fish farm in Spain. Turbot larvae, post-larvae (0.2 g) and juveniles (5 and 15 g) were experimentally infected....

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Bibliographic Details
Published in:Journal of Fish Diseases
Main Authors: Villamil, L., Figueras Huerta, Antonio, Toranzo, A. E., Planas, Miguel, Novoa, Beatriz
Format: Article in Journal/Newspaper
Language:English
Published: Wiley-Blackwell 2003
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Online Access:http://hdl.handle.net/10261/56346
https://doi.org/10.1046/j.1365-2761.2003.00461.x
Description
Summary:11 páginas, 7 figuras, 2 tablas A bacterial strain, characterized as Vibrio pelagius (Hq 222), was isolated from a turbot, Scophthalmus maximus (L.), larvae mass mortality in a commercial fish farm in Spain. Turbot larvae, post-larvae (0.2 g) and juveniles (5 and 15 g) were experimentally infected. The bacterium appeared to be very virulent for larvae and post-larvae, LD50 being <5 bacteria mL−1 for larvae 1 week post-infection and 3.9 × 105 bacteria mL−1 in post-larvae at day 12 post-infection. The bacterial strain was recovered in pure culture from the internal organs of infected fish. Histological lesions in post-larvae exhibited swelling and necrosis of gill secondary lamellae, sloughing of intestinal mucosa and necrosis of haematopoietic tissue in the kidney. Vibrio pelagius (Hq 222) was able to grow in sterile sea water when incubated at room temperature or at 15 °C. Vibrio pelagius (Hq 222) was more adherent to the turbot cell lines TV-1 and TF than Escherichia coli. In both cell lines, the number of adhered bacteria increased with incubation time This work was funded by Project 1FD97-044-C03- 03 from FEDER funds. Peer reviewed