Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index
Hemodilution of bone marrow (BM) aspirates is a limitation of multiparameter flow cytometry (MFC) in plasma cell disorders. There is a need for a validated approach for assessing sample quality and the distribution of non-plasma cell BM populations by MFC could provide a solution. We evaluated BM-as...
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Format: | Article in Journal/Newspaper |
Language: | English |
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Springer Nature
2023
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Online Access: | http://hdl.handle.net/10261/347175 https://doi.org/10.1038/s41408-023-00951-2 https://doi.org/10.13039/100005189 https://doi.org/10.13039/501100000781 https://doi.org/10.13039/501100001840 https://doi.org/10.13039/100003887 https://doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85178181238 |
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ftcsic:oai:digital.csic.es:10261/347175 |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
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English |
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Cancer microenvironment Myeloma |
spellingShingle |
Cancer microenvironment Myeloma Óskarsson, Jón Þórir Rögnvaldsson, Sæmundur Thorsteinsdottir, Sigrun Aspelund, Thor Gunnarsson, Steinar Bragi Hákonardóttir, Guðlaug Katrín Sigurðardóttir, Guðrún Ásta Þórðardóttir, Ásdís Rósa Gíslason, Gauti Kjartan Ólafsson, Andri Sigurðsson, Jón Kristinn Eyþórsson, Elías Jónsson, Ásbjörn Viðarsson, Brynjar Önundarson, Páll Torfi Agnarsson, Bjarni A Pálmason, Róbert Sigurðardóttir, Margrét Þorsteinsdóttir, Ingunn Ólafsson, Ísleifur Harding, Stephen Flores-Montero, Juan Orfao, Alberto Durie, Brian G. M. Love, Thorvardur Jon Kristinsson, Sigurdur Yngvi Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index |
topic_facet |
Cancer microenvironment Myeloma |
description |
Hemodilution of bone marrow (BM) aspirates is a limitation of multiparameter flow cytometry (MFC) in plasma cell disorders. There is a need for a validated approach for assessing sample quality and the distribution of non-plasma cell BM populations by MFC could provide a solution. We evaluated BM-associated cell populations, assessed by next-generation flow cytometry (NGF) and white blood cell (WBC) count in 351 BM aspirated samples from 219 participants with plasma cell disorders in the Iceland Screens, Treats, or Prevents MM study (iStopMM), as markers of hemodilution by their discriminatory ability between first and (generally more hemodiluted) second pull BM aspirated samples. The most discriminating markers were used to derive a novel BM quality index (BMQI). Nucleated red blood cells and myeloid precursors provided the greatest discriminatory ability between first vs second pull samples (area under the curve (AUC): 0.87 and 0.85, respectively), significantly better than B cell precursors (AUC = 0.64; p < 0.001), mast cells (AUC = 0.65; p < 0.001), and the BM WBC count (AUC = 0.77; p < 0.05). We generated a novel BMQI that is intrinsic to current NGF protocols, for evaluating quality of diagnostic BM samples and suggest the use of a BMQI scoring system for interpreting results and guiding appropriate actions. The iStopMM study is funded by the Black Swan Research Initiative by the International Myeloma Foundation and the Icelandic Centre for Research (grant agreement numbers 173857, 217897-051, and 228521-051). Furthermore, this project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement numbers 716677 and 101045549). The project has also received funding from The Leukemia & Lymphoma Society (grant number 2340-23) and the International Myeloma Society. Screening tests are performed by The Binding Site Ltd., Birmingham, UK. Additional funding is provided by the University of Iceland, ... |
author2 |
International Myeloma Foundation Icelandic Centre for Research European Research Council European Commission Leukemia & Lymphoma Society (US) University of Iceland Icelandic Cancer Society |
format |
Article in Journal/Newspaper |
author |
Óskarsson, Jón Þórir Rögnvaldsson, Sæmundur Thorsteinsdottir, Sigrun Aspelund, Thor Gunnarsson, Steinar Bragi Hákonardóttir, Guðlaug Katrín Sigurðardóttir, Guðrún Ásta Þórðardóttir, Ásdís Rósa Gíslason, Gauti Kjartan Ólafsson, Andri Sigurðsson, Jón Kristinn Eyþórsson, Elías Jónsson, Ásbjörn Viðarsson, Brynjar Önundarson, Páll Torfi Agnarsson, Bjarni A Pálmason, Róbert Sigurðardóttir, Margrét Þorsteinsdóttir, Ingunn Ólafsson, Ísleifur Harding, Stephen Flores-Montero, Juan Orfao, Alberto Durie, Brian G. M. Love, Thorvardur Jon Kristinsson, Sigurdur Yngvi |
author_facet |
Óskarsson, Jón Þórir Rögnvaldsson, Sæmundur Thorsteinsdottir, Sigrun Aspelund, Thor Gunnarsson, Steinar Bragi Hákonardóttir, Guðlaug Katrín Sigurðardóttir, Guðrún Ásta Þórðardóttir, Ásdís Rósa Gíslason, Gauti Kjartan Ólafsson, Andri Sigurðsson, Jón Kristinn Eyþórsson, Elías Jónsson, Ásbjörn Viðarsson, Brynjar Önundarson, Páll Torfi Agnarsson, Bjarni A Pálmason, Róbert Sigurðardóttir, Margrét Þorsteinsdóttir, Ingunn Ólafsson, Ísleifur Harding, Stephen Flores-Montero, Juan Orfao, Alberto Durie, Brian G. M. Love, Thorvardur Jon Kristinsson, Sigurdur Yngvi |
author_sort |
Óskarsson, Jón Þórir |
title |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index |
title_short |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index |
title_full |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index |
title_fullStr |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index |
title_full_unstemmed |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index |
title_sort |
determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: proposal for a bone marrow quality index |
publisher |
Springer Nature |
publishDate |
2023 |
url |
http://hdl.handle.net/10261/347175 https://doi.org/10.1038/s41408-023-00951-2 https://doi.org/10.13039/100005189 https://doi.org/10.13039/501100000781 https://doi.org/10.13039/501100001840 https://doi.org/10.13039/100003887 https://doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85178181238 |
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Iceland |
genre_facet |
Iceland |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/ 716677 info:eu-repo/grantAgreement/EC/HE/101045549 Blood cancer journal Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at 10.1038/s41408-023-00951-2 https://doi.org/10.1038/s41408-023-00951-2 Sí Blood Cancer Journal 13 (2023) http://hdl.handle.net/10261/347175 doi:10.1038/s41408-023-00951-2 2044-5385 http://dx.doi.org/10.13039/100005189 http://dx.doi.org/10.13039/501100000781 http://dx.doi.org/10.13039/501100001840 http://dx.doi.org/10.13039/100003887 http://dx.doi.org/10.13039/501100000780 38040702 2-s2.0-85178181238 https://api.elsevier.com/content/abstract/scopus_id/85178181238 |
op_rights |
open |
op_doi |
https://doi.org/10.1038/s41408-023-00951-210.13039/10000518910.13039/50110000078110.13039/50110000184010.13039/10000388710.13039/501100000780 |
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1802645922030551040 |
spelling |
ftcsic:oai:digital.csic.es:10261/347175 2024-06-23T07:54:00+00:00 Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index Óskarsson, Jón Þórir Rögnvaldsson, Sæmundur Thorsteinsdottir, Sigrun Aspelund, Thor Gunnarsson, Steinar Bragi Hákonardóttir, Guðlaug Katrín Sigurðardóttir, Guðrún Ásta Þórðardóttir, Ásdís Rósa Gíslason, Gauti Kjartan Ólafsson, Andri Sigurðsson, Jón Kristinn Eyþórsson, Elías Jónsson, Ásbjörn Viðarsson, Brynjar Önundarson, Páll Torfi Agnarsson, Bjarni A Pálmason, Róbert Sigurðardóttir, Margrét Þorsteinsdóttir, Ingunn Ólafsson, Ísleifur Harding, Stephen Flores-Montero, Juan Orfao, Alberto Durie, Brian G. M. Love, Thorvardur Jon Kristinsson, Sigurdur Yngvi International Myeloma Foundation Icelandic Centre for Research European Research Council European Commission Leukemia & Lymphoma Society (US) University of Iceland Icelandic Cancer Society 2023-12-01 application/pdf http://hdl.handle.net/10261/347175 https://doi.org/10.1038/s41408-023-00951-2 https://doi.org/10.13039/100005189 https://doi.org/10.13039/501100000781 https://doi.org/10.13039/501100001840 https://doi.org/10.13039/100003887 https://doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85178181238 en eng Springer Nature #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/ 716677 info:eu-repo/grantAgreement/EC/HE/101045549 Blood cancer journal Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at 10.1038/s41408-023-00951-2 https://doi.org/10.1038/s41408-023-00951-2 Sí Blood Cancer Journal 13 (2023) http://hdl.handle.net/10261/347175 doi:10.1038/s41408-023-00951-2 2044-5385 http://dx.doi.org/10.13039/100005189 http://dx.doi.org/10.13039/501100000781 http://dx.doi.org/10.13039/501100001840 http://dx.doi.org/10.13039/100003887 http://dx.doi.org/10.13039/501100000780 38040702 2-s2.0-85178181238 https://api.elsevier.com/content/abstract/scopus_id/85178181238 open Cancer microenvironment Myeloma artículo http://purl.org/coar/resource_type/c_6501 2023 ftcsic https://doi.org/10.1038/s41408-023-00951-210.13039/10000518910.13039/50110000078110.13039/50110000184010.13039/10000388710.13039/501100000780 2024-05-29T00:06:20Z Hemodilution of bone marrow (BM) aspirates is a limitation of multiparameter flow cytometry (MFC) in plasma cell disorders. There is a need for a validated approach for assessing sample quality and the distribution of non-plasma cell BM populations by MFC could provide a solution. We evaluated BM-associated cell populations, assessed by next-generation flow cytometry (NGF) and white blood cell (WBC) count in 351 BM aspirated samples from 219 participants with plasma cell disorders in the Iceland Screens, Treats, or Prevents MM study (iStopMM), as markers of hemodilution by their discriminatory ability between first and (generally more hemodiluted) second pull BM aspirated samples. The most discriminating markers were used to derive a novel BM quality index (BMQI). Nucleated red blood cells and myeloid precursors provided the greatest discriminatory ability between first vs second pull samples (area under the curve (AUC): 0.87 and 0.85, respectively), significantly better than B cell precursors (AUC = 0.64; p < 0.001), mast cells (AUC = 0.65; p < 0.001), and the BM WBC count (AUC = 0.77; p < 0.05). We generated a novel BMQI that is intrinsic to current NGF protocols, for evaluating quality of diagnostic BM samples and suggest the use of a BMQI scoring system for interpreting results and guiding appropriate actions. The iStopMM study is funded by the Black Swan Research Initiative by the International Myeloma Foundation and the Icelandic Centre for Research (grant agreement numbers 173857, 217897-051, and 228521-051). Furthermore, this project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement numbers 716677 and 101045549). The project has also received funding from The Leukemia & Lymphoma Society (grant number 2340-23) and the International Myeloma Society. Screening tests are performed by The Binding Site Ltd., Birmingham, UK. Additional funding is provided by the University of Iceland, ... Article in Journal/Newspaper Iceland Digital.CSIC (Spanish National Research Council) |