A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles

We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species Crassostrea gigas (Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (HPO, KHPO, and KHPO), P/bCCP mol...

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Bibliographic Details
Main Authors: Fernández Penas, Raquel, Verdugo-Escamilla, Cristóbal, Triunfo, Carla, Gärtner, S., D'Urso, Annarita, Oltolina, Francesca, Follenzi, Antonia, Maoloni, Gabriele, Cölfen, Helmut, Falini, Giuseppe, Gómez-Morales, Jaime
Other Authors: Ministerio de Ciencia e Innovación (España), European Commission
Format: Article in Journal/Newspaper
Language:unknown
Published: Royal Society of Chemistry (UK) 2023
Subjects:
Crassostrea gigas
Online Access:http://hdl.handle.net/10261/342532
https://doi.org/10.1039/d3tb00856h
https://doi.org/10.13039/501100000780
https://doi.org/10.13039/501100004837
Description
Summary:We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species Crassostrea gigas (Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (HPO, KHPO, and KHPO), P/bCCP molar ratios (0.24, 0.6, and 0.96), digestion temperatures (25-200 °C), and digestion times (1 week-2 months) on the transformation process was thoroughly investigated. At 1 week, the minimum temperature to yield the full transformation significantly reduced from 160 °C to 120 °C when using KHPO instead of KHPO at a P/bCCP ratio of 0.6, and even to 80 °C at a P/bCCP ratio of 0.96. The transformation took place via a dissolution-reprecipitation mechanism driven by the favorable balance between HA precipitation and bCCP dissolution, due to the lower solubility product of HA than that of calcite at any of the tested temperatures. Both the bCCP and the derived HA particles were cytocompatible for MG-63 human osteosarcoma cells and m17.ASC murine mesenchymal stem cells, and additionally, they promoted the osteogenic differentiation of m17.ASC, especially the HA particles. Because of their physicochemical features and biological compatibility, both particles could be useful osteoinductive platforms for translational applications in bone tissue engineering. This work was supported by project PCI2020-112108 funded by MCI/AEI/10.13039/501100011033 (Spain) and the European Union ‘‘NextGenerationEU’’/PRTR’’. PCI2020-112108 is part of the CASEAWA project of the ERA-NET Cofund BlueBio Programme, supported by the European Union (H2020), (Grant Agreement ERA-NET no. 817992).

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