Fungal Endophytes Enhance the Photoprotective Mechanisms and Photochemical Efficiency in the Antarctic Colobanthus quitensis (Kunth) Bartl. Exposed to UV-B Radiation

• Published in: Frontiers in Ecology and Evolution
• Main Authors: Barrera, Andrea, Hereme, Rasme, Ruíz-Lara, Simón, Larrondo, Luis Fernando, Gundel, Pedro E., Pollmann, Stephan, Molina-Montenegro, Marco A., Ramos, Patricio
• Other Authors: Instituto Antártico Chileno, Universidad de Talca, Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
• Format: Article in Journal/Newspaper
• Language: English
• Published: Frontiers Media 2020
• Subjects: Antarctica; Colobanthus quitensis; Flavonols; Fungal endophytes; Molecular response; UV-B stress; Antarctic; Inach; The Antarctic; Antarc*
• Online Access: http://hdl.handle.net/10261/340762; https://doi.org/10.3389/fevo.2020.00122; https://doi.org/10.13039/501100002850; https://doi.org/10.13039/501100010599; https://api.elsevier.com/content/abstract/scopus_id/85085166606

13 Pág. Antarctic plants have developed mechanisms to deal with one or more adverse factors which allow them to successfully survive such extreme environment. Certain effective mechanisms to face adverse stress factors can arise from the establishment of functional symbiosis with endophytic fungi. In this work, we explored the role of fungal endophytes on host plant performance under high level of UV-B radiation, a harmful factor known to damage structure and function of cell components. In order to unveil the underlying mechanisms, we characterized the expression of genes associated to UV-B photoreception, accumulation of key flavonoids, and physiological responses of Colobanthus quitensis plants with (E+) and without (E−) fungal endophytes, under contrasting levels of UV-B radiation. The deduced proteins of CqUVR8, CqHY5, and CqFLS share the characteristic domains and display high degrees of similarity with other corresponding proteins in plants. Endophyte symbiotic plants showed lower lipid peroxidation and higher photosynthesis efficiency under high UV-B radiation. In comparison with E−, E+ plants showed lower CqUVR8, CqHY5, and CqFLS transcript levels. The content of quercetin, a ROS-scavenger flavonoid, in leaves of E- plants exposed to high UV-B was almost 8-fold higher than that in E+ plants 48 h after treatment. Our results suggest that endophyte fungi minimize cell damage and boost physiological performance in the Antarctic plants increasing the tolerance to UV-B radiation. Fungal endophytes appear as fundamental biological partners for plants to cope with the highly damaging UV-B radiation of Antarctica. We acknowledge the financial, authorization and logistic support of the Chilean Antarctic Institute (INACH). MM-M was supported by project FONDECYT 1181034 and PII 20150126. REDES #190078 from Conicyt. PR acknowledges “Núcleo Científico Multidisciplinario” from Universidad de Talca. This article contributes to the SCAR biological research programs “Antarctic Thresholds - Ecosystem Resilience and ...