Inhibition of the infection of several fish viruses of distinct families with probable serineand low-pH-dependent entry by NK-lysin

• Main Authors: Falcó, Alberto, Adamek, Mikolaj, Bello Pérez, Melissa, Pereiro, Patricia, Mas, Vicente, Novoa, Beatriz, Encinar, José Antonio
• Other Authors: Ministerio de Ciencia e Innovación (España), Ministerio de Agricultura, Pesca y Alimentación (España), European Maritime, Fisheries and Aquaculture Fund, German Research Foundation
• Format: Still Image
• Language: English
• Published: 2022
• Subjects: Scophthalmus maximus; Turbot
• Online Access: http://hdl.handle.net/10261/283661; https://doi.org/10.13039/501100004837; https://doi.org/10.13039/501100001659

Poster.-- XVI Congreso Nacional de Virología, Málaga, 6-9 de septiembre de 2022 NK-lysins are a family of antimicrobial peptides conserved in all vertebrate groups that stand out for their antitumor and broad-spectrum antimicrobial activity. The tertiary structure of these peptides shows helical regions stabilized by disulfide bridges that exhibit a lysine-rich facet responsible for its interaction with anionic lipid membranes. In our previous papers, the peptide corresponding to such region in the turbot (Scophthalmus maximus) NK-lysin ortholog (Nkl71-100) showed membrane-disrupting activity against the parasite Philasterides dicentrarchi, as well as inhibition of the pH-dependent fusion step of the entry phase of the spring viremia of carp virus (SVCV). Biophysical data showed that Nkl71-100 preferentially interacts with anionic phosphatidylserine lipid bilayers at low pH conditions. In the present study, the susceptibility of a diverse range of fish viruses to Nkl71-100 has been determined in vitro. In particular, SVCV and tilapia lake virus (TiLV) showed high sensitivity to the Nkl71-100 treatment, while the inhibitory effect on cyprinid herpesvirus 3 (CyHV-3) was only present at higher concentrations and common carp paramyxovirus (CCPV) infectivity was not affected by the peptide. In conclusion, the fusion step of the viral entry phase is postulated as a promising target for the treatment of some viruses with high economic impact on aquaculture by means of molecules such as NK-lysins Grant RTI2018-101969-J-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”; Project MetDisFish funded by the Ministry of Agriculture, Fisheries and Food (MAPA) and European Maritime, Fisheries and Aquaculture Fund (EMFAF); project 426513195 funded by the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) No