Molecular basis of ocean acidification sensitivity and adaptation in Mytilus galloprovincialis

• Published in: iScience
• Main Authors: Kapsenberg, Lydia, Bitter, Mark C., Miglioli, Angelica, Aparicio-Estalella, Clàudia, Pelejero, Carles, Gattuso, Jean-Pierre, Dumollard, Rémi
• Other Authors: National Science Foundation (US), European Commission, The University of Chicago Center in Paris, Department of Education (US), Agence Nationale de la Recherche (France), Università degli studi di Genova, Agencia Estatal de Investigación (España)
• Format: Article in Journal/Newspaper
• Language: English
• Published: Cell Press 2022
• Subjects: Ocean acidification
• Online Access: http://hdl.handle.net/10261/279347; https://doi.org/10.1016/j.isci.2022.104677; https://doi.org/10.13039/501100000780; https://doi.org/10.13039/100000001; https://doi.org/10.13039/501100004702; https://doi.org/10.13039/501100001665; https://doi.org/10.13039/501100011033; https://doi.org/10.13039/100000138

17 pages, 3 figures, 1 table, supplemental information https://doi.org/10.1016/j.isci.2022.104677.-- Data and code availability: • RNA-seq data have been deposited at NCBI and are publicly available (BioProject number is listed in the key resources table). DNA-seq data have been deposited at NCBI and have publicly been available since the publication of Bitter et al. (2019) (BioProject number is listed in the key resources table). • All original code has been deposited at GitHub and is publicly available (URL is listed in the key resources table). • Any additional information required to reanalyze the data reported in this paper is available from the Lead contact upon request Predicting the potential for species adaption to climate change is challenged by the need to identify the physiological mechanisms that underpin species vulnerability. Here, we investigated the sensitivity to ocean acidification in marine mussels during early development, and specifically the trochophore stage. Using RNA and DNA sequencing and in situ RNA hybridization, we identified developmental processes associated with abnormal development and rapid adaptation to low pH. Trochophores exposed to low pH seawater exhibited 43 differentially expressed genes. Gene annotation and in situ hybridization of differentially expressed genes point to pH sensitivity of (1) shell field development and (2) cellular stress response. Five genes within these two processes exhibited shifts in allele frequencies indicative of a potential for rapid adaptation. This case study contributes direct evidence that protecting species’ existing genetic diversity is a critical management action to facilitate species resilience to climate change This research was funded by the US National Science Foundation (NSF; OCE-1521597 to L.K.) and European Commission Horizon 2020 Marie Skłodowska-Curie Action (No. 747637 to L.K.). Additionally, the DNA sequencing data used in this study, and originally published by Bitter et al. (2019), were generated, in part, through ...