Enzymatic production of ecodiesel by using a commercial lipase calb, immobilized by physical adsorption on mesoporous organosilica materials

• Published in: Catalysts
• Main Authors: Luna, Carlos, Gascón Pérez, Victoria, López-Tenllado, F.J., Bautista, Felipa M., Verdugo-Escamilla, Cristóbal, Aguado-Deblas, Laura, Calero, Juan, Romero, Antonio A., Luna, Diego, Estévez, Rafael
• Other Authors: Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), European Commission, Junta de Andalucía
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Multidisciplinary Digital Publishing Institute 2021
• Subjects: Biofuel; Ecodiesel; Biodiesel; Commercial CALB lipase; Ordered mesoporous materials (PMO); Amorphous siliceous material MS3030; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10261/270117; https://doi.org/10.3390/catal11111350; https://doi.org/10.13039/501100000780; https://doi.org/10.13039/501100011011; https://doi.org/10.13039/501100004837; https://doi.org/10.13039/501100003329

The synthesis of two biocatalysts based on a commercial Candida antarctica lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (octyl-MS3030). The maximum enzyme load was 122 mg enzyme/g support on PMO and 288 mg enzyme/g support on octyl-MS3030. In addition, the biocatalytic efficiency was corroborated by two reaction tests based on the hydrolysis of p-nitrophenylacetate (p-NPA) and tributyrin (TB). The transesterification of sunflower oil with ethanol was carried out over the bio-catalysts synthesized at the following reaction conditions: 6 mL sunflower oil, 1.75 mL EtOH, 30 °C, 25 μL NaOH 10 N and 300 rpm, attaining conversion values over 80% after 3 h of reaction time. According to the results obtained, we can confirm that these biocatalytic systems are viable candi-dates to develop, optimize and improve a new methodology to achieve the integration of glycerol in different monoacylglycerol molecules together with fatty acid ethyl esters (FAEE) molecules to obtain Ecodiesel. This research is supported by MINECO-ENE2016-81013-R (AEI/FEDER, EU), MICIIN (Project ref. PID2019-104953RB-100), Consejería de Transformación Económica, Industria, Conocimiento y Universidades de la Junta de Andalucía (UCO-FEDER Project CATOLIVAL, ref. 1264113-R, 2018, and Project ref. P18-RT-4822) and FEDER Funds for financial support. The Government of Ireland Postdoctoral Fellowship Programme-2015 (GOIPD/2015/287) is thankfully acknowledge by V.G.-P