Brains in metamorphosis. Spatiotemporal transcriptome dynamics in hatchery-reared flatfishes
XIII Congress of the Iberian Association of Comparative Endocrinology - AIEC (online), 16-17 September 2021 Metamorphosis is a captivating process of change by which the morphology of the larva is reshaped into a completely different one to face the new challenges related to adult life. In fish, inc...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Conference Object |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10261/260745 https://doi.org/10.13039/501100003329 |
| Summary: | XIII Congress of the Iberian Association of Comparative Endocrinology - AIEC (online), 16-17 September 2021 Metamorphosis is a captivating process of change by which the morphology of the larva is reshaped into a completely different one to face the new challenges related to adult life. In fish, including paradigmatic species with commercial value such as flatfish, this process initiated in the brain has traditionally been considered to be a critical rearing point and despite the pioneering molecular work carried out on the metamorphosis of the Japanese flounder and Atlantic Halibut the underlying molecular basis of flatfish metamorphosis is still relatively poorly characterized. The key point for understanding this phenomenon is to know how the information encoded in the DNA is accurately used by cells to perform the physiological functions that are required during the different phases of metamorphic remodeling process. We already know that the access to this information is tightly regulated by an intricated system of different regulatory layers. These determine, for instance, which genes need to be turned on or off at different developmental times. So, the tight regulation of gene expression in time and space is crucial to understand how the complexity and variation within an organism can arise from a relatively simple DNA blueprint. We investigated the whole-genome transcriptional profile of turbot brain at three key points in their metamorphic remodeling process (pre-metamorphic, climax and post-metamorphic). We found that gene expression dynamics are largest at pre and post metamorphic stages. Biological pathway enrichment analysis revealed that genes more highly expressed at metamorphosis climax are associated with innate immune system, while genes more highly expressed in pre-metamorphic and post-metamorphic stages are associated with cell differentiation and oxygen carrier activity, respectively. Additionally, our results confirm the importance of the thyroid-stimulating hormone in the metamorphosis ... |
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