Enzymatic preparation of structured triacylglycerides containing γ-linolenic acid
Structured triacylglycerides (STAG) rich in γ-linolenic acid (GLA) were prepared using a three-step process. First, with the addition of molecular sieves (MS) at different addition times (3, 9, 12 and 15 h) and loads (2.5, 5, 10 and 15%, based on the total weight of substrates), the enrichment of GL...
| Published in: | Biocatalysis and Agricultural Biotechnology |
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| Main Authors: | , , , , , |
| Other Authors: | |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Elsevier
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10261/228629 https://doi.org/10.1016/j.bcab.2020.101680 https://doi.org/10.13039/501100003141 |
| Summary: | Structured triacylglycerides (STAG) rich in γ-linolenic acid (GLA) were prepared using a three-step process. First, with the addition of molecular sieves (MS) at different addition times (3, 9, 12 and 15 h) and loads (2.5, 5, 10 and 15%, based on the total weight of substrates), the enrichment of GLA from evening primrose oil (EPO) and 1-butanol (BtOH) was improved via Candida rugosa lipase-catalysed esterification reactions. Secondly, the GLA-enriched fraction was separated by thin-layer chromatography (TLC) to be further set to react during the third step in the presence of glycerol and Candida antarctica fraction B (CALB), under different enzyme loadings (5, 10, 15 and 20%, based on the total weight of substrates), temperatures (30, 40, 50 and 60 °C) and substrates molar ratios (1:1, 2:1, 3:1 and 4:1, GLA:glycerol). 60% of STAG containing 49 wt% of GLA were produced by using 15% of CALB at 60 °C and a 3:1 molar ratio. Author López-Martínez gratefully acknowledges financial support for his MSc studies (scholarship no. 328716) from the National Council of Science and Technology (CONACYT) of Mexico. Peer reviewed |
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