Absorption of Anisakis spp. proteins using human Caco-2 cellular model
Trabajo presentado a la 49th West European Fish Technologists Association (WEFTA), celebrada en Tórshavn (Islas Feroe) del 15 al 17 de octubre de 2019. The risk of exposure Anisakis spp. allergens is mainly through the digestive tract, its pathogenic potential depends not only on its resistance to g...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | , |
| Format: | Conference Object |
| Language: | unknown |
| Published: |
2019
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10261/203926 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100000780 |
| Summary: | Trabajo presentado a la 49th West European Fish Technologists Association (WEFTA), celebrada en Tórshavn (Islas Feroe) del 15 al 17 de octubre de 2019. The risk of exposure Anisakis spp. allergens is mainly through the digestive tract, its pathogenic potential depends not only on its resistance to gastrointestinal digestion but also on its ability to cross the barrier of the intestinal epithelium. Previous studies have demonstrated the resistance to gastrointestinal digestion of allergens, mainly low molecular weight (unpublished data). The aim of this piece of work was to study the ability to cross the intestinal epithelial barrier of Anisakis spp. antigens/allergens from Anisakis crude extract (CE) using human Caco-2 cellular model. The integrity of the Caco-2 cells monolayer was characterised measuring the transepithelial electrical resistance (TEER) and paracellular permeability and the analysis of the antigens/allergens transported through the epithelium was performed by SDS-PAGE and immunoblotting. The analysis of TEER shows a significant decrease after two hours in samples with Anisakis CE added. Immunodetection assays (WB) show that the passage of the allergen Ani s 4 might combine paracellular and transcellular transport. Regarding the paracellular permeability evaluated by Lucifer Yellow, the results obtained after incubation of cells with CE, support that the Anisakis CE compromises the integrity of the Caco-2 monolayer. Viability assays show there is not any cytotoxic effect. Putative mechanisms underlying the decrease in TEER have been analysed (protease and phosphatase inhibition, reactive oxygen species determination). However, we have not currently identified the mechanism involved and further studies will be performed. Associated to the TEER changes, we have observed disorganization of occludin by confocal microscopy. Anisakis allergens are able to cross the intestinal barrier, which would justify the presence of symptoms in some patients sensitized to Anisakis ssp. after the consumption of ... |
|---|