Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles
Trabajo presentado a la 49th West European Fish Technologists Association (WEFTA) Conference celebrada en Tórshavn (Faroe Islands) del 15 al 17 de octubre de 2019. Protein by-products from Stripped weakfish (Cynoscion guatucupa) were hydrolysated (5% degree of hydrolysis) using Alcalase or Protamex....
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ftcsic:oai:digital.csic.es:10261/203505 2024-02-11T10:03:42+01:00 Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles Oliveira Lima, Karina López Caballero, M. E. Alemán, Ailén Fernández de Palencia, P. Gómez Guillén, M. C. Prentice, Carlos Montero García, Pilar Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) European Commission Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil) 2019 http://hdl.handle.net/10261/203505 https://doi.org/10.13039/501100002322 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100000780 en eng #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-52825-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84161-C2 AGL2017-84161-C2/AEI/10.13039/501100011033 Publisher's version Sí 49th WEFTA Conference (2019) http://hdl.handle.net/10261/203505 http://dx.doi.org/10.13039/501100002322 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 open póster de congreso http://purl.org/coar/resource_type/c_6670 2019 ftcsic https://doi.org/10.13039/50110000232210.13039/50110001103310.13039/50110000332910.13039/501100000780 2024-01-16T10:49:52Z Trabajo presentado a la 49th West European Fish Technologists Association (WEFTA) Conference celebrada en Tórshavn (Faroe Islands) del 15 al 17 de octubre de 2019. Protein by-products from Stripped weakfish (Cynoscion guatucupa) were hydrolysated (5% degree of hydrolysis) using Alcalase or Protamex. The cytotoxicity, and its antioxidant and ACE-inhibitory activities before and after a simulated in vitro gastrointestinal digestion, as well as its bioaccessibility, were determined. Both hydrolysates had similar values of 2,2´-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity (ABTS), although Protamex hydrolysate attained higher values in ferric-reducing antioxidant power (RP) and greater antihypertensive capacity. After digestion (4 h), ABTS values remained constant. However, RP values decreased over time, which was reflected in lower bioaccessibility. Cell line assays of Caco2 showed that hydrolysates were not cytotoxic. Then, the chitosan nanoparticles loaded with Protamex hydrolysate was carried out in order to protect it from a possible loss of activity and to be able to control release. The size of the resultant particles were < 200 nm, potential Z >30 mV with encapsulation efficiency of 43%. As for the antioxidant activity (ABTS), the results revealed a gradual and controlled release of the peptide from the nanoparticle, which varied according to the amount of crosslinking agent. Likewise, the hydrolysate maintained its antihypertensive activity once nanoencapsulated. In order to evaluate their potential application, the nanoparticles were incorporated into a model emulsion or a real mayonnaise-type system. Emulsions with nanoparticles retain a higher lipid content at the pH of the emulsion (3.3), and to a greater extent at pH 7.5, 37ºC (intestinal conditions), while an increase in the release of peptides from charged nanoparticles was also observed. In the real system (mayonnaise), the nanoparticles conferred a good consistency, with similar stability and less lipid ... Still Image Faroe Islands Digital.CSIC (Spanish National Research Council) Faroe Islands Tórshavn ENVELOPE(-6.772,-6.772,62.010,62.010) |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
Trabajo presentado a la 49th West European Fish Technologists Association (WEFTA) Conference celebrada en Tórshavn (Faroe Islands) del 15 al 17 de octubre de 2019. Protein by-products from Stripped weakfish (Cynoscion guatucupa) were hydrolysated (5% degree of hydrolysis) using Alcalase or Protamex. The cytotoxicity, and its antioxidant and ACE-inhibitory activities before and after a simulated in vitro gastrointestinal digestion, as well as its bioaccessibility, were determined. Both hydrolysates had similar values of 2,2´-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity (ABTS), although Protamex hydrolysate attained higher values in ferric-reducing antioxidant power (RP) and greater antihypertensive capacity. After digestion (4 h), ABTS values remained constant. However, RP values decreased over time, which was reflected in lower bioaccessibility. Cell line assays of Caco2 showed that hydrolysates were not cytotoxic. Then, the chitosan nanoparticles loaded with Protamex hydrolysate was carried out in order to protect it from a possible loss of activity and to be able to control release. The size of the resultant particles were < 200 nm, potential Z >30 mV with encapsulation efficiency of 43%. As for the antioxidant activity (ABTS), the results revealed a gradual and controlled release of the peptide from the nanoparticle, which varied according to the amount of crosslinking agent. Likewise, the hydrolysate maintained its antihypertensive activity once nanoencapsulated. In order to evaluate their potential application, the nanoparticles were incorporated into a model emulsion or a real mayonnaise-type system. Emulsions with nanoparticles retain a higher lipid content at the pH of the emulsion (3.3), and to a greater extent at pH 7.5, 37ºC (intestinal conditions), while an increase in the release of peptides from charged nanoparticles was also observed. In the real system (mayonnaise), the nanoparticles conferred a good consistency, with similar stability and less lipid ... |
author2 |
Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) European Commission Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil) |
format |
Still Image |
author |
Oliveira Lima, Karina López Caballero, M. E. Alemán, Ailén Fernández de Palencia, P. Gómez Guillén, M. C. Prentice, Carlos Montero García, Pilar |
spellingShingle |
Oliveira Lima, Karina López Caballero, M. E. Alemán, Ailén Fernández de Palencia, P. Gómez Guillén, M. C. Prentice, Carlos Montero García, Pilar Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles |
author_facet |
Oliveira Lima, Karina López Caballero, M. E. Alemán, Ailén Fernández de Palencia, P. Gómez Guillén, M. C. Prentice, Carlos Montero García, Pilar |
author_sort |
Oliveira Lima, Karina |
title |
Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles |
title_short |
Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles |
title_full |
Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles |
title_fullStr |
Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles |
title_full_unstemmed |
Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles |
title_sort |
biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles |
publishDate |
2019 |
url |
http://hdl.handle.net/10261/203505 https://doi.org/10.13039/501100002322 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100000780 |
long_lat |
ENVELOPE(-6.772,-6.772,62.010,62.010) |
geographic |
Faroe Islands Tórshavn |
geographic_facet |
Faroe Islands Tórshavn |
genre |
Faroe Islands |
genre_facet |
Faroe Islands |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-52825-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84161-C2 AGL2017-84161-C2/AEI/10.13039/501100011033 Publisher's version Sí 49th WEFTA Conference (2019) http://hdl.handle.net/10261/203505 http://dx.doi.org/10.13039/501100002322 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 |
op_rights |
open |
op_doi |
https://doi.org/10.13039/50110000232210.13039/50110001103310.13039/50110000332910.13039/501100000780 |
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1790600022110240768 |