Biological activity of hydrolysates from industrial by-products encapsulated in chitosan nanoparticles

• Main Authors: Oliveira Lima, Karina, López Caballero, M. E., Alemán, Ailén, Fernández de Palencia, P., Gómez Guillén, M. C., Prentice, Carlos, Montero García, Pilar
• Other Authors: Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
• Format: Still Image
• Language: English
• Published: 2019
• Subjects: Faroe Islands; Tórshavn
• Online Access: http://hdl.handle.net/10261/203505; https://doi.org/10.13039/501100002322; https://doi.org/10.13039/501100011033; https://doi.org/10.13039/501100003329; https://doi.org/10.13039/501100000780

Trabajo presentado a la 49th West European Fish Technologists Association (WEFTA) Conference celebrada en Tórshavn (Faroe Islands) del 15 al 17 de octubre de 2019. Protein by-products from Stripped weakfish (Cynoscion guatucupa) were hydrolysated (5% degree of hydrolysis) using Alcalase or Protamex. The cytotoxicity, and its antioxidant and ACE-inhibitory activities before and after a simulated in vitro gastrointestinal digestion, as well as its bioaccessibility, were determined. Both hydrolysates had similar values of 2,2´-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity (ABTS), although Protamex hydrolysate attained higher values in ferric-reducing antioxidant power (RP) and greater antihypertensive capacity. After digestion (4 h), ABTS values remained constant. However, RP values decreased over time, which was reflected in lower bioaccessibility. Cell line assays of Caco2 showed that hydrolysates were not cytotoxic. Then, the chitosan nanoparticles loaded with Protamex hydrolysate was carried out in order to protect it from a possible loss of activity and to be able to control release. The size of the resultant particles were < 200 nm, potential Z >30 mV with encapsulation efficiency of 43%. As for the antioxidant activity (ABTS), the results revealed a gradual and controlled release of the peptide from the nanoparticle, which varied according to the amount of crosslinking agent. Likewise, the hydrolysate maintained its antihypertensive activity once nanoencapsulated. In order to evaluate their potential application, the nanoparticles were incorporated into a model emulsion or a real mayonnaise-type system. Emulsions with nanoparticles retain a higher lipid content at the pH of the emulsion (3.3), and to a greater extent at pH 7.5, 37ºC (intestinal conditions), while an increase in the release of peptides from charged nanoparticles was also observed. In the real system (mayonnaise), the nanoparticles conferred a good consistency, with similar stability and less lipid ...