| Summary: | Trabajo presentado en la 22nd International Conference of Zoology, celebrado en Okinawa (Japón), del 14 al 19 de noviembre de 2016 In teleosts, recombinant gonadotropins (rGTHs), both in vitro and in vivo, have been able to induce the steroidogenesis and gonad development, however the in vivo results have been variable. In vivo, specific rGTH stimulated estradiol (E2) production in female of species such as orange-spotted grouper (Epinehelus coioides) or rosy bitterling (Rhodeus ocellatus), achieving ovulation in the latter, in that study however, already sexually matured females were used. In males, rGTHs were also able to induce the androgenesis process e.g. in zebrafish (Danio rerio) and European sea bass (Dicentrarchus labrax). Generally, the hormonal treatment was not able to induce the spermiation in vivo, with the exception of goldfish (Carassius auratus) and European sea bass, where the treated fish were already sexually matured at the beginning of the study. In Japanese eel (Anguilla japonica), recombinant FSH stimulated the in vitro production of E2 and testosterone in oocytes, but only at vitellogenic. In vivo, rGTHs did not stimulate an increase in the gonadosomatic index in females, but some induction of vitellogenesis was observed. In males, rGTHs expressed by baculovirus, yeasts or Drosophila cell lines were able to induce androgenesis in vitro. A complete spermatogenesis, with the presence of some spermatozoa in the testis, was achieved with rGTHs expressed by baculovirus. In vivo, Japanese eel and goldfish rGTHs, expressed by baculovirus, stimulated a complete spermatogenesis in male eels, but spermiation has never been achieved. Therefore, in the current work, the aim was to check the functionality in vivo of specific rGTHs in European eel. European eel males (n=72; 100.1±1.9 g) from a local fish farm were distributed in four 150-L aquaria and progressively adapted to sea water and 20 ºC. Single-chain recombinant FSH and LH were obtained by transfection of a mammalian cell line with further ...
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