Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids
Enzyme catalyzed interesterification (EIE) of pine seed oil (PSO) and a fully hydrogenated soybean oil (FHSBO) were studied in batch reactors in solvent-free media to prepare different semisolid fats rich in polyunsaturated fatty acids (PUFA). Optimal operation conditions found were: 10 % (w/w) enzy...
Published in: | Journal of the American Oil Chemists' Society |
---|---|
Main Authors: | , , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
John Wiley & Sons
2013
|
Subjects: | |
Online Access: | http://hdl.handle.net/10261/181792 https://doi.org/10.1007/s11746-012-2149-0 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/501100007273 |
id |
ftcsic:oai:digital.csic.es:10261/181792 |
---|---|
record_format |
openpolar |
spelling |
ftcsic:oai:digital.csic.es:10261/181792 2024-02-11T09:57:24+01:00 Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids Otero Hernández, Cristina Márquez, Pablo Criado, Manuel Hernández-Martín, Estela Consejo Superior de Investigaciones Científicas (España) Comisión Interministerial de Ciencia y Tecnología, CICYT (España) 2013-01 http://hdl.handle.net/10261/181792 https://doi.org/10.1007/s11746-012-2149-0 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/501100007273 unknown John Wiley & Sons https://doi.org/10.1007/s11746-012-2149-0 Sí JAOCS, Journal of the American Oil Chemists' Society 90(1): 81-90 (2013) 0003-021X http://hdl.handle.net/10261/181792 doi:10.1007/s11746-012-2149-0 1558-9331 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100007273 none Structured lipids Zero trans fats Acyl migration Pinolenic acid Pine seed oil Interesterification Semi‐solid fat Lipase Δ‐5 fatty acid Polyunsaturated fatty acids artículo http://purl.org/coar/resource_type/c_6501 2013 ftcsic https://doi.org/10.1007/s11746-012-2149-010.13039/50110000333910.13039/501100007273 2024-01-16T10:39:13Z Enzyme catalyzed interesterification (EIE) of pine seed oil (PSO) and a fully hydrogenated soybean oil (FHSBO) were studied in batch reactors in solvent-free media to prepare different semisolid fats rich in polyunsaturated fatty acids (PUFA). Optimal operation conditions found were: 10 % (w/w) enzyme loading, 75 C and magnetic agitation at 300 rpm. Quasi-equilibrium conditions were reached after 2, 3 and 6 h, when immobilized lipases from Thermomyces lanuginosus (Lipozyme® TL IM), Candida antarctica B. (Novozym® 435) and Rhizomucor miehei (Lipozyme® RM IM) from Novozymes A/S (Bagsvaerd, Denmark) were employed, respectively. Similar distributions of unsaturated to saturated fatty acid (UFA/SFA) residues along the glycerol backbone of the fat products were obtained with both non-selective and sn-1(3) regioselective lipases due to significant spontaneous acyl migration during the reaction. The products had higher UFA/SFA ratios at the sn-2 position (2.4-2.5, 1.4-1.7, and 0.5-0.8 for the trials involving 20, 40 and 70 % FHSBO, w/w, respectively) than the corresponding physical blends (0.8, 0.4 and 0.5, respectively). Fat products containing 3.1-11.6 % (w/w) pinolenic acid (Pn) and 16.1-35.7 % (w/w) linoleic acid (L) at the sn-2 position were prepared. The free acid contents of EIE products prepared with Lipozyme ® TL IM and Novozym® 435 were 6.1-6.4 and 2.5-2.6, respectively. Residual activities of Lipozyme® TL IM and Novozym® 435 diminish by ca. 20 % after 9 reaction cycles. This work was supported by the Spanish CICYT (MAT2007‐6666‐C02‐02). A predoctoral fellowship for E. Hernández‐Martín was provided by the Spanish Research Council, CSIC. Peer Reviewed Article in Journal/Newspaper Antarc* Antarctica Digital.CSIC (Spanish National Research Council) Eie ENVELOPE(7.983,7.983,63.033,63.033) Journal of the American Oil Chemists' Society 90 1 81 90 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Structured lipids Zero trans fats Acyl migration Pinolenic acid Pine seed oil Interesterification Semi‐solid fat Lipase Δ‐5 fatty acid Polyunsaturated fatty acids |
spellingShingle |
Structured lipids Zero trans fats Acyl migration Pinolenic acid Pine seed oil Interesterification Semi‐solid fat Lipase Δ‐5 fatty acid Polyunsaturated fatty acids Otero Hernández, Cristina Márquez, Pablo Criado, Manuel Hernández-Martín, Estela Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids |
topic_facet |
Structured lipids Zero trans fats Acyl migration Pinolenic acid Pine seed oil Interesterification Semi‐solid fat Lipase Δ‐5 fatty acid Polyunsaturated fatty acids |
description |
Enzyme catalyzed interesterification (EIE) of pine seed oil (PSO) and a fully hydrogenated soybean oil (FHSBO) were studied in batch reactors in solvent-free media to prepare different semisolid fats rich in polyunsaturated fatty acids (PUFA). Optimal operation conditions found were: 10 % (w/w) enzyme loading, 75 C and magnetic agitation at 300 rpm. Quasi-equilibrium conditions were reached after 2, 3 and 6 h, when immobilized lipases from Thermomyces lanuginosus (Lipozyme® TL IM), Candida antarctica B. (Novozym® 435) and Rhizomucor miehei (Lipozyme® RM IM) from Novozymes A/S (Bagsvaerd, Denmark) were employed, respectively. Similar distributions of unsaturated to saturated fatty acid (UFA/SFA) residues along the glycerol backbone of the fat products were obtained with both non-selective and sn-1(3) regioselective lipases due to significant spontaneous acyl migration during the reaction. The products had higher UFA/SFA ratios at the sn-2 position (2.4-2.5, 1.4-1.7, and 0.5-0.8 for the trials involving 20, 40 and 70 % FHSBO, w/w, respectively) than the corresponding physical blends (0.8, 0.4 and 0.5, respectively). Fat products containing 3.1-11.6 % (w/w) pinolenic acid (Pn) and 16.1-35.7 % (w/w) linoleic acid (L) at the sn-2 position were prepared. The free acid contents of EIE products prepared with Lipozyme ® TL IM and Novozym® 435 were 6.1-6.4 and 2.5-2.6, respectively. Residual activities of Lipozyme® TL IM and Novozym® 435 diminish by ca. 20 % after 9 reaction cycles. This work was supported by the Spanish CICYT (MAT2007‐6666‐C02‐02). A predoctoral fellowship for E. Hernández‐Martín was provided by the Spanish Research Council, CSIC. Peer Reviewed |
author2 |
Consejo Superior de Investigaciones Científicas (España) Comisión Interministerial de Ciencia y Tecnología, CICYT (España) |
format |
Article in Journal/Newspaper |
author |
Otero Hernández, Cristina Márquez, Pablo Criado, Manuel Hernández-Martín, Estela |
author_facet |
Otero Hernández, Cristina Márquez, Pablo Criado, Manuel Hernández-Martín, Estela |
author_sort |
Otero Hernández, Cristina |
title |
Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids |
title_short |
Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids |
title_full |
Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids |
title_fullStr |
Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids |
title_full_unstemmed |
Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids |
title_sort |
enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids |
publisher |
John Wiley & Sons |
publishDate |
2013 |
url |
http://hdl.handle.net/10261/181792 https://doi.org/10.1007/s11746-012-2149-0 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/501100007273 |
long_lat |
ENVELOPE(7.983,7.983,63.033,63.033) |
geographic |
Eie |
geographic_facet |
Eie |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://doi.org/10.1007/s11746-012-2149-0 Sí JAOCS, Journal of the American Oil Chemists' Society 90(1): 81-90 (2013) 0003-021X http://hdl.handle.net/10261/181792 doi:10.1007/s11746-012-2149-0 1558-9331 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100007273 |
op_rights |
none |
op_doi |
https://doi.org/10.1007/s11746-012-2149-010.13039/50110000333910.13039/501100007273 |
container_title |
Journal of the American Oil Chemists' Society |
container_volume |
90 |
container_issue |
1 |
container_start_page |
81 |
op_container_end_page |
90 |
_version_ |
1790609691874689024 |