Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids

Enzyme catalyzed interesterification (EIE) of pine seed oil (PSO) and a fully hydrogenated soybean oil (FHSBO) were studied in batch reactors in solvent-free media to prepare different semisolid fats rich in polyunsaturated fatty acids (PUFA). Optimal operation conditions found were: 10 % (w/w) enzy...

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Published in:Journal of the American Oil Chemists' Society
Main Authors: Otero Hernández, Cristina, Márquez, Pablo, Criado, Manuel, Hernández-Martín, Estela
Other Authors: Consejo Superior de Investigaciones Científicas (España), Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Format: Article in Journal/Newspaper
Language:unknown
Published: John Wiley & Sons 2013
Subjects:
Eie
Online Access:http://hdl.handle.net/10261/181792
https://doi.org/10.1007/s11746-012-2149-0
https://doi.org/10.13039/501100003339
https://doi.org/10.13039/501100007273
id ftcsic:oai:digital.csic.es:10261/181792
record_format openpolar
spelling ftcsic:oai:digital.csic.es:10261/181792 2024-02-11T09:57:24+01:00 Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids Otero Hernández, Cristina Márquez, Pablo Criado, Manuel Hernández-Martín, Estela Consejo Superior de Investigaciones Científicas (España) Comisión Interministerial de Ciencia y Tecnología, CICYT (España) 2013-01 http://hdl.handle.net/10261/181792 https://doi.org/10.1007/s11746-012-2149-0 https://doi.org/10.13039/501100003339 https://doi.org/10.13039/501100007273 unknown John Wiley & Sons https://doi.org/10.1007/s11746-012-2149-0 Sí JAOCS, Journal of the American Oil Chemists' Society 90(1): 81-90 (2013) 0003-021X http://hdl.handle.net/10261/181792 doi:10.1007/s11746-012-2149-0 1558-9331 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100007273 none Structured lipids Zero trans fats Acyl migration Pinolenic acid Pine seed oil Interesterification Semi‐solid fat Lipase Δ‐5 fatty acid Polyunsaturated fatty acids artículo http://purl.org/coar/resource_type/c_6501 2013 ftcsic https://doi.org/10.1007/s11746-012-2149-010.13039/50110000333910.13039/501100007273 2024-01-16T10:39:13Z Enzyme catalyzed interesterification (EIE) of pine seed oil (PSO) and a fully hydrogenated soybean oil (FHSBO) were studied in batch reactors in solvent-free media to prepare different semisolid fats rich in polyunsaturated fatty acids (PUFA). Optimal operation conditions found were: 10 % (w/w) enzyme loading, 75 C and magnetic agitation at 300 rpm. Quasi-equilibrium conditions were reached after 2, 3 and 6 h, when immobilized lipases from Thermomyces lanuginosus (Lipozyme® TL IM), Candida antarctica B. (Novozym® 435) and Rhizomucor miehei (Lipozyme® RM IM) from Novozymes A/S (Bagsvaerd, Denmark) were employed, respectively. Similar distributions of unsaturated to saturated fatty acid (UFA/SFA) residues along the glycerol backbone of the fat products were obtained with both non-selective and sn-1(3) regioselective lipases due to significant spontaneous acyl migration during the reaction. The products had higher UFA/SFA ratios at the sn-2 position (2.4-2.5, 1.4-1.7, and 0.5-0.8 for the trials involving 20, 40 and 70 % FHSBO, w/w, respectively) than the corresponding physical blends (0.8, 0.4 and 0.5, respectively). Fat products containing 3.1-11.6 % (w/w) pinolenic acid (Pn) and 16.1-35.7 % (w/w) linoleic acid (L) at the sn-2 position were prepared. The free acid contents of EIE products prepared with Lipozyme ® TL IM and Novozym® 435 were 6.1-6.4 and 2.5-2.6, respectively. Residual activities of Lipozyme® TL IM and Novozym® 435 diminish by ca. 20 % after 9 reaction cycles. This work was supported by the Spanish CICYT (MAT2007‐6666‐C02‐02). A predoctoral fellowship for E. Hernández‐Martín was provided by the Spanish Research Council, CSIC. Peer Reviewed Article in Journal/Newspaper Antarc* Antarctica Digital.CSIC (Spanish National Research Council) Eie ENVELOPE(7.983,7.983,63.033,63.033) Journal of the American Oil Chemists' Society 90 1 81 90
institution Open Polar
collection Digital.CSIC (Spanish National Research Council)
op_collection_id ftcsic
language unknown
topic Structured lipids
Zero trans fats
Acyl migration
Pinolenic acid
Pine seed oil
Interesterification
Semi‐solid fat
Lipase
Δ‐5 fatty acid
Polyunsaturated fatty acids
spellingShingle Structured lipids
Zero trans fats
Acyl migration
Pinolenic acid
Pine seed oil
Interesterification
Semi‐solid fat
Lipase
Δ‐5 fatty acid
Polyunsaturated fatty acids
Otero Hernández, Cristina
Márquez, Pablo
Criado, Manuel
Hernández-Martín, Estela
Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids
topic_facet Structured lipids
Zero trans fats
Acyl migration
Pinolenic acid
Pine seed oil
Interesterification
Semi‐solid fat
Lipase
Δ‐5 fatty acid
Polyunsaturated fatty acids
description Enzyme catalyzed interesterification (EIE) of pine seed oil (PSO) and a fully hydrogenated soybean oil (FHSBO) were studied in batch reactors in solvent-free media to prepare different semisolid fats rich in polyunsaturated fatty acids (PUFA). Optimal operation conditions found were: 10 % (w/w) enzyme loading, 75 C and magnetic agitation at 300 rpm. Quasi-equilibrium conditions were reached after 2, 3 and 6 h, when immobilized lipases from Thermomyces lanuginosus (Lipozyme® TL IM), Candida antarctica B. (Novozym® 435) and Rhizomucor miehei (Lipozyme® RM IM) from Novozymes A/S (Bagsvaerd, Denmark) were employed, respectively. Similar distributions of unsaturated to saturated fatty acid (UFA/SFA) residues along the glycerol backbone of the fat products were obtained with both non-selective and sn-1(3) regioselective lipases due to significant spontaneous acyl migration during the reaction. The products had higher UFA/SFA ratios at the sn-2 position (2.4-2.5, 1.4-1.7, and 0.5-0.8 for the trials involving 20, 40 and 70 % FHSBO, w/w, respectively) than the corresponding physical blends (0.8, 0.4 and 0.5, respectively). Fat products containing 3.1-11.6 % (w/w) pinolenic acid (Pn) and 16.1-35.7 % (w/w) linoleic acid (L) at the sn-2 position were prepared. The free acid contents of EIE products prepared with Lipozyme ® TL IM and Novozym® 435 were 6.1-6.4 and 2.5-2.6, respectively. Residual activities of Lipozyme® TL IM and Novozym® 435 diminish by ca. 20 % after 9 reaction cycles. This work was supported by the Spanish CICYT (MAT2007‐6666‐C02‐02). A predoctoral fellowship for E. Hernández‐Martín was provided by the Spanish Research Council, CSIC. Peer Reviewed
author2 Consejo Superior de Investigaciones Científicas (España)
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
format Article in Journal/Newspaper
author Otero Hernández, Cristina
Márquez, Pablo
Criado, Manuel
Hernández-Martín, Estela
author_facet Otero Hernández, Cristina
Márquez, Pablo
Criado, Manuel
Hernández-Martín, Estela
author_sort Otero Hernández, Cristina
title Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids
title_short Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids
title_full Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids
title_fullStr Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids
title_full_unstemmed Enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids
title_sort enzymatic interesterification between pine seed oil and a hydrogenated fat to prepare semi-solid fats rich in pinolenic acid and other polyunsaturated fatty acids
publisher John Wiley & Sons
publishDate 2013
url http://hdl.handle.net/10261/181792
https://doi.org/10.1007/s11746-012-2149-0
https://doi.org/10.13039/501100003339
https://doi.org/10.13039/501100007273
long_lat ENVELOPE(7.983,7.983,63.033,63.033)
geographic Eie
geographic_facet Eie
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_relation https://doi.org/10.1007/s11746-012-2149-0

JAOCS, Journal of the American Oil Chemists' Society 90(1): 81-90 (2013)
0003-021X
http://hdl.handle.net/10261/181792
doi:10.1007/s11746-012-2149-0
1558-9331
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100007273
op_rights none
op_doi https://doi.org/10.1007/s11746-012-2149-010.13039/50110000333910.13039/501100007273
container_title Journal of the American Oil Chemists' Society
container_volume 90
container_issue 1
container_start_page 81
op_container_end_page 90
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