Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)
Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecul...
Published in: | Journal of Helminthology |
---|---|
Main Authors: | , , , , , , , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Cambridge University Press
2016
|
Subjects: | |
Online Access: | http://hdl.handle.net/10261/156308 https://doi.org/10.1017/S0022149X15000218 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100003339 |
Summary: | Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed. This study was funded by the Spanish Ministry of Economy and Competiveness and the European Regional Development Fund Projects CGL2004-01348, POL2006-05175, CGL2007-60369 and CTM2011-24427. V.V. was supported by a PhD grant from the Spanish Council of Scientific Research (CSIC) and the European Social Fund (JAEPre08-01053).M.J.P.was supported by a PhDgrant from the Spanish Ministry of Science and Innovation (BES2005-8465). Peer Reviewed |
---|