Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT...
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American Chemical Society
2016
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Online Access: | http://hdl.handle.net/10261/150109 https://doi.org/10.1021/acs.jproteome.6b00154 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100012818 |
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ftcsic:oai:digital.csic.es:10261/150109 2024-02-11T10:03:23+01:00 Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS Valdés, Alberto Bergquist, Jonas García-Cañas, Virginia Cifuentes, Alejandro Swedish Research Council Ministerio de Economía y Competitividad (España) Comunidad de Madrid 2016 http://hdl.handle.net/10261/150109 https://doi.org/10.1021/acs.jproteome.6b00154 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100012818 unknown American Chemical Society #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-53609-P S2013/ABI-2728/ALIBIRD-CM Sí doi:10.1021/acs.jproteome.6b00154 e-issn: 1535-3907 issn: 1535-3893 Journal of Proteome Research 15(6): 1971-1985 (2016) http://hdl.handle.net/10261/150109 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 none Rosemary extract Antiproliferative activity Quantitative proteomics Mass spectrometry HT-29 cells Colon cancer Dimethyl labeling Foodomics artículo http://purl.org/coar/resource_type/c_6501 2016 ftcsic https://doi.org/10.1021/acs.jproteome.6b0015410.13039/50110000332910.13039/100012818 2024-01-16T10:23:19Z In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The major objective of this study was to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols. Using this methodology, 1909 and 698 proteins were identified and quantified in cell extracts. The polyphenol-enriched rosemary extract treatment changed the expression of several proteins in a time- and concentration-dependent manner. Most of the altered proteins are implicated in the activation of Nrf2 transcription factor and the unfolded protein response. In conclusion, rosemary polyphenols induced proteomic changes that were related to the attenuation of aggresome formation and activation of autophagy to alleviate cellular stress. This work was supported by the projects AGL2014-53609-P (Ministerio de Economia y Competitividad, Spain) and S2013/ABI-2728 (Comunidad de Madrid). A.V. thanks the Ministerio de Economia y Competitividad for his FPI predoctoral fellowship (BES-2012-057014). The Swedish Research Council (2011-4423 and 2015-4870; J.B.) is acknowledged for financial support. Peer Reviewed Article in Journal/Newspaper DML Digital.CSIC (Spanish National Research Council) Journal of Proteome Research 15 6 1971 1985 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Rosemary extract Antiproliferative activity Quantitative proteomics Mass spectrometry HT-29 cells Colon cancer Dimethyl labeling Foodomics |
spellingShingle |
Rosemary extract Antiproliferative activity Quantitative proteomics Mass spectrometry HT-29 cells Colon cancer Dimethyl labeling Foodomics Valdés, Alberto Bergquist, Jonas García-Cañas, Virginia Cifuentes, Alejandro Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS |
topic_facet |
Rosemary extract Antiproliferative activity Quantitative proteomics Mass spectrometry HT-29 cells Colon cancer Dimethyl labeling Foodomics |
description |
In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The major objective of this study was to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols. Using this methodology, 1909 and 698 proteins were identified and quantified in cell extracts. The polyphenol-enriched rosemary extract treatment changed the expression of several proteins in a time- and concentration-dependent manner. Most of the altered proteins are implicated in the activation of Nrf2 transcription factor and the unfolded protein response. In conclusion, rosemary polyphenols induced proteomic changes that were related to the attenuation of aggresome formation and activation of autophagy to alleviate cellular stress. This work was supported by the projects AGL2014-53609-P (Ministerio de Economia y Competitividad, Spain) and S2013/ABI-2728 (Comunidad de Madrid). A.V. thanks the Ministerio de Economia y Competitividad for his FPI predoctoral fellowship (BES-2012-057014). The Swedish Research Council (2011-4423 and 2015-4870; J.B.) is acknowledged for financial support. Peer Reviewed |
author2 |
Swedish Research Council Ministerio de Economía y Competitividad (España) Comunidad de Madrid |
format |
Article in Journal/Newspaper |
author |
Valdés, Alberto Bergquist, Jonas García-Cañas, Virginia Cifuentes, Alejandro |
author_facet |
Valdés, Alberto Bergquist, Jonas García-Cañas, Virginia Cifuentes, Alejandro |
author_sort |
Valdés, Alberto |
title |
Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS |
title_short |
Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS |
title_full |
Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS |
title_fullStr |
Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS |
title_full_unstemmed |
Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS |
title_sort |
comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap ms/ms |
publisher |
American Chemical Society |
publishDate |
2016 |
url |
http://hdl.handle.net/10261/150109 https://doi.org/10.1021/acs.jproteome.6b00154 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/100012818 |
genre |
DML |
genre_facet |
DML |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-53609-P S2013/ABI-2728/ALIBIRD-CM Sí doi:10.1021/acs.jproteome.6b00154 e-issn: 1535-3907 issn: 1535-3893 Journal of Proteome Research 15(6): 1971-1985 (2016) http://hdl.handle.net/10261/150109 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 |
op_rights |
none |
op_doi |
https://doi.org/10.1021/acs.jproteome.6b0015410.13039/50110000332910.13039/100012818 |
container_title |
Journal of Proteome Research |
container_volume |
15 |
container_issue |
6 |
container_start_page |
1971 |
op_container_end_page |
1985 |
_version_ |
1790599602092638208 |