Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS

In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT...

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Bibliographic Details
Published in:Journal of Proteome Research
Main Authors: Valdés, Alberto, Bergquist, Jonas, García-Cañas, Virginia, Cifuentes, Alejandro
Other Authors: Swedish Research Council, Ministerio de Economía y Competitividad (España), Comunidad de Madrid
Format: Article in Journal/Newspaper
Language:unknown
Published: American Chemical Society 2016
Subjects:
Rosemary extract
Antiproliferative activity
Quantitative proteomics
Mass spectrometry
HT-29 cells
Colon cancer
Dimethyl labeling
Foodomics
DML
Online Access:http://hdl.handle.net/10261/150109
https://doi.org/10.1021/acs.jproteome.6b00154
https://doi.org/10.13039/501100003329
https://doi.org/10.13039/100012818
Description
Summary:In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The major objective of this study was to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols. Using this methodology, 1909 and 698 proteins were identified and quantified in cell extracts. The polyphenol-enriched rosemary extract treatment changed the expression of several proteins in a time- and concentration-dependent manner. Most of the altered proteins are implicated in the activation of Nrf2 transcription factor and the unfolded protein response. In conclusion, rosemary polyphenols induced proteomic changes that were related to the attenuation of aggresome formation and activation of autophagy to alleviate cellular stress. This work was supported by the projects AGL2014-53609-P (Ministerio de Economia y Competitividad, Spain) and S2013/ABI-2728 (Comunidad de Madrid). A.V. thanks the Ministerio de Economia y Competitividad for his FPI predoctoral fellowship (BES-2012-057014). The Swedish Research Council (2011-4423 and 2015-4870; J.B.) is acknowledged for financial support. Peer Reviewed

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