Detection of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in wild small mammals in Spain

• Main Authors: Gómez, Paula, González-Barrio, David, Benito, Daniel, García, Jesús T., Viñuela, Javier, Zarazaga, Myriam, Ruiz Fons, Francisco, Torres, Carmen
• Format: Still Image
• Language: unknown
• Published: 2014
• Subjects: Microtus arvalis
• Online Access: http://hdl.handle.net/10261/146174

Resumen del póster presentado al 24th European Congress of Clinical Microbiology and Infectious Diseases, celebrado en Barcelona (España) del 10 al 13 de mayo de 2014. [Objectives]: To determine the rate of Staphylococcus aureus fecal carriage in wild small mammals recovered in Spain and to perform the genetic characterization of obtained isolates. [Methods]: Fecal samples of 101 small mammals (54 Microtus arvalis, 29 Apodemus sylvaticus, 6 Mus spretus, 6 Rattus norvegicus, 5 Crocidura russula, and 1 Eliomys quercinus) were recovered in two different Spanish localities (74 north-central, 27 southern). Wild mammals were captured by researchers at IREC during 2011-2013. Samples were suspended in saline solution and were inoculated in BHI with 6.5% NaCl; later, 100µl was seeded on Manitol-Salt Agar (MSA) and ORSAB plates. Isolates were identified by microbiological and molecular methods (PCR of nuc gene). Antibiotic susceptibility profile was determined by disk-diffusion method for 17 antibiotics. The presence of resistance genes mecA, mecC (mecALGA251), blaZ and blaZ allotype, as well as the detection of SCCmecXI were studied by PCR. Production of ß-lactamase was Objectives. To determine the rate of Staphylococcus aureus fecal carriage in wild small mammals recovered in Spain and to perform the genetic characterization of obtained isolates. [Methods]: Fecal samples of 101 small mammals (54 Microtus arvalis, 29 Apodemus sylvaticus, 6 Mus spretus, 6 Rattus norvegicus, 5 Crocidura russula, and 1 Eliomys quercinus) were recovered in two different Spanish localities (74 north-central, 27 southern). Wild mammals were captured by researchers at IREC during 2011-2013. Samples were suspended in saline solution and were inoculated in BHI with 6.5% NaCl; later, 100µl was seeded on Manitol-Salt Agar (MSA) and ORSAB plates. Isolates were identified by microbiological and molecular methods (PCR of nuc gene). Antibiotic susceptibility profile was determined by disk-diffusion method for 17 antibiotics. The presence of ...