| Summary: | Resumen del trabajo presentado a la VI International Conference on Environmental, Industrial and Applied Microbiology, celebrada en Barcelona (España) del 28 al 30 de octubre de 2015.-- et al. Spleen samples collected in 1994–2013 in Spain from 213 wild carnivores belonging to five different families were analysed using real time PCR. Canine protoparvovirus-1 infection was confirmed in 18% of samples, chiefly in wolves (Canis lupus). Nearly all of the VP2 gene in the 19 positive cases was sequenced and 15 different nucleotide sequence types, clustered into eight amino-acid sequence types (aaSTs), were detected. Most were identical or closely related to sequences previously found in domestic and captive animals. Fifteen isolates including five aaSTs corresponded to the canine parvovirus (CPV) 2c, which was identified in the majority of the wolves and badgers (Meles meles) analyzed, as well as in a genet (Genetta genetta) and a wildcat (Felis silvestris), the latter animal sampled before this strain had been detected in dogs in Spain. Identical CPV-2c sequences were found in five individuals belonging to three different families sampled in 2001–2013 in two distant regions. Sequences from three wolves corresponding to CPV-2b clustered near the CPV-2c group into a phylogenetic group that includes sequences from a stone marten (Martes foina) from Portugal and North American carnivores, which provides further evidence for the existence of an intermediate clade between CPV-2b and CPV-2c. CPV-2b infection was confirmed in a fox (Vulpes vulpes) and probably in a stone marten; as well, the feline panleukopenia virus was detected in a stone marten, a badger and probably in a genet. Our findings expand the range of hosts for parvoviruses, reveal the predominance of CPV-2c among European wildlife, and confirm that virus transmission occurs between wild and domestic carnivores. Resumen del trabajo presentado a la VI International Conference on Environmental, Industrial and Applied Microbiology, celebrada en Barcelona ...
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