A novel CalB-type lipase discovered by fungal genomes mining
11 p.-4 fig.-1 tab. The fungus Pseudozyma antarctica produces a lipase (CalB) with broad substrate specificity, stability, high regio- and enantio-selectivity. It is active in non-aqueous organic solvents and at elevated temperatures. Hence, CalB is a robust biocatalyst for chemical conversions on a...
Published in: | PLOS ONE |
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Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science
2015
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Subjects: | |
Online Access: | http://hdl.handle.net/10261/116781 https://doi.org/10.1371/journal.pone.0124882 |
Summary: | 11 p.-4 fig.-1 tab. The fungus Pseudozyma antarctica produces a lipase (CalB) with broad substrate specificity, stability, high regio- and enantio-selectivity. It is active in non-aqueous organic solvents and at elevated temperatures. Hence, CalB is a robust biocatalyst for chemical conversions on an industrial scale. Here we report the in silico mining of public metagenomes and fungal genomes to discover novel lipases with high homology to CalB. The candidates were selected taking into account homology and conserved motifs criteria, as well as, phylogeny and 3D model analyses. The most promising candidate (PlicB) presented interesting structural properties. PlicB was expressed in a heterologous host, purified and partially characterized. Further experiments will allow finding novel catalytic properties with biotechnological interest. This work was supported by the Spanish projects BIO2012-36372, RTC-2014-1777-3 and S2013/MAE-2907. Peer reviewed |
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