A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined to HPAEC-PAD
An optimized method is presented to determine free (DFCHO) and combined monosaccharides (CCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination between desalination with electro-dialysis and high performance anion exchange chromatography coupled to p...
| Main Authors: | , , , |
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| Format: | Text |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://doi.org/10.5194/os-2020-2 https://www.ocean-sci-discuss.net/os-2020-2/ |
| Summary: | An optimized method is presented to determine free (DFCHO) and combined monosaccharides (CCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination between desalination with electro-dialysis and high performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Free neutral sugars, such as glucose and galactose, were found with 95–98 % recovery rates. Free amino sugars and uronic acids were strongly depleted during electro-dialysis at pH = 8, but an adjustment of the pH could result in higher recoveries (58–59 % for amino sugars at pH = 11; 45–49 % for uronic acids at pH = 1.5). The applicability of this method for the analysis of CCHO was evaluated with standard solution and real seawater samples compared with another established desalination method using membrane dialysis. DFCHO in real field samples from different regions on earth ranged between 11–118 nM and CCHO between 260–1410 nM. This novel method potentially contributes to a better understanding of biogeochemical processes in the oceans and sea-air transfer processes of organic matter into the atmosphere during further research studies. |
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