Selectivity of R-alpha-monobenzoate glycerol synthesis catalyzed by Candida antarctica lipase B immobilized on heterofunctional supports

• Published in: Process Biochemistry
• Main Authors: Guajardo, Nadia, Bernal, C, Wilson, Lorena, Cabrera-Muñoz, Zaida Tamara
• Format: Article in Journal/Newspaper
• Language: English
• Subjects: Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10533/219740; https://doi.org/10.1016/j.procbio.2015.06.025

The main goal of this work was to study different strategies of immobilization of Candida antarctica lipase B (CALB) in order to evaluate changes in the selectivity of this enzyme when R-alpha-monobenzoate glycerol (R-alpha-MBG) is obtained through of asymmetric esterification. CALB was immobilized on sepharose and silica functionalized with octyl groups (monofunctional supports) and undecanol-glyoxyl and octyl-epoxide groups (heterofunctional supports). Our results showed that the enzyme could be immobilized by all carriers, with activity yields ranging from 52% to 83%. CALB immobilized on silica octyl was the most active (367 IU g(biocatalyst)(-1)) with immobilization yields in terms of protein and expressed activity of 72% and 50% respectively and exhibited a higher half-life in 100% 1,4-dioxane at 50 degrees C (85,000 h). In contrast, CALB immobilized on heterofunctional silica support was the most selective biocatalyst, reaching an enantiomeric excess of 99% of R-alpha-MBG in 100% 1,4-dioxane. In terms of configuration the sepharose biocatalysts results in a S-enantiomer, while silica biocatalysts results in a R-enantiomer. The catalysis of asymmetric esterification of glycerol with benzoic acid to obtain R-alpha-MBG by CALB immobilized in heterofunctional silica is highly selective and, to our knowledge, is the most selective reported to date. (C) 2015 Elsevier Ltd. All rights reserved.