Isolation, purification and preliminary characterization of cryophilic proteases of marine origin

The isolation and preliminary characterization of a trypsin-like protease with high activity at 20 degreesC is described. This protease was isolated from Antarctic krill (Euphasia superba) by a two-step chromatography process and the use of zymogram analyses. The protease has a molecular weight of 3...

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Bibliographic Details
Main Authors: Asenjo-De Leuze, Juan, Andrews-Farrow, Barbara, Barria , C., Salamanca, M.
Format: Article in Journal/Newspaper
Language:Spanish
Published: KLUWER ACADEMIC PUBL
Subjects:
Antarctic
Antarc*
Antarctic Krill
Online Access:http://hdl.handle.net/10533/197643
https://doi.org/10.1023/A:1016383212244
Description
Summary:The isolation and preliminary characterization of a trypsin-like protease with high activity at 20 degreesC is described. This protease was isolated from Antarctic krill (Euphasia superba) by a two-step chromatography process and the use of zymogram analyses. The protease has a molecular weight of 30 kDa and a pI of 4.1. Its specific activity at 20 degreesC on BAPNA is 0.5 U/mg.

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