Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant

Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Rivas Aravena, Andrea, Fuentes, Jazmin, Cartagena, Julio, Brito, Tania, Poggio, Thelma Veronica, la Torre, Jose Leonardo, Mendoza, Hegaly, Gonzalez Nilo, Fernando Danilo, Sandino, Ana María, Eugenio, Emilio Osvaldo
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier
Subjects:
Nanoparticles
Adjuvant
Vaccine
Atlantic Salmon
Salmon Alphavirus
Isav
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
Argentina
Torre
Aravena
Atlantic salmon
Online Access:http://hdl.handle.net/11336/4151
Description
Summary:Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection. Fil: Rivas Aravena, Andrea. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile. Comision Chilena de Energía Nuclear. Departamento de Aplicaciones Nucleares; Chile Fil: Fuentes, Jazmin. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile Fil: Cartagena, Julio. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile Fil: Brito, Tania. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile Fil: Poggio, Thelma Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina. Tecnovax; Argentina Fil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar ...

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