Molecular recognition of an acyl-enzyme intermediate on the lipase B from Candida antarctica

This investigation provides evidences of the acyl enzyme species involved in theinteraction of R/S-ketoprofen with the lipase B from Candida antarctica. The interaction between the profen and the enzyme was studied by in situ Time-Resolved ATR-FTIR both in static and transient conditions. Particular...

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Bibliographic Details
Published in:Catalysis Science & Technology
Main Authors: Toledo, Victoria, Llerena Suster, Carlos Rafael, Ferreira, María Luján, Collins, Sebastián Enrique, Briand, Laura Estefania
Format: Article in Journal/Newspaper
Language:English
Published: Royal Society of Chemistry
Subjects:
Acyl-Enzyme
Lipase
Infrared Spectroscopy
R/S-Ketoprofen
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
Argentina
Toledo
Ferreira
Briand
Antarc*
Antarctica
Online Access:http://hdl.handle.net/11336/31389
Description
Summary:This investigation provides evidences of the acyl enzyme species involved in theinteraction of R/S-ketoprofen with the lipase B from Candida antarctica. The interaction between the profen and the enzyme was studied by in situ Time-Resolved ATR-FTIR both in static and transient conditions. Particularly, modulation excitation spectroscopy (MES) with phase sensitive detection (PSD) allowed to univocally distinguishing the signals belonging to the interaction between ketoprofen and the enzyme from the strong background signals. These experimental tools coupled with theoretical DFT analysis allowed to propose various species due to the interaction of ketoprofen with serine through H bonding (without reaction) and the acyl enzyme species (ester bond formation) that are the intermediates in the biocatalytic assisted esterification andhydrolysis using lipases. Fil: Toledo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas Argentina Fil: Llerena Suster, Carlos Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas Argentina Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Collins, Sebastián Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Briand, Laura Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en ...

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