Cold active transglutaminase from antarctic Penicillium chrysogenum: Partial purification, characterization and potential application in food technology

• Published in: Biocatalysis and Agricultural Biotechnology
• Main Authors: Glodowsky, Alejandro Pablo, Ruberto, Lucas Adolfo Mauro, Martorell, María Martha, Mac Cormack, Walter Patricio, Levin, Gustavo Javier
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier
• Subjects: ANTARCTIC FUNGI; COLD-ACTIVE ENZYME; FOOD TECHNOLOGY; TRANSGLUTAMINASE; https://purl.org/becyt/ford/2.9; https://purl.org/becyt/ford/2; Antarctic; Argentino; Argentina; Pablo; Entre Ríos; Antarc*; Dirección Nacional del Antártico; Instituto Antártico Argentino
• Online Access: http://hdl.handle.net/11336/131169

A novel transglutaminase (TGase, EC 2.3.2.13) from antarctic Penicillium chrysogenum was partially purified using ammonium sulfate precipitation and anionic exchange chromatography. The final extract had a specific activity of 7.81 mU/mg, purification fold of 7.16, and a yield of 5.02%. The molecula...