Using Xenon as a Heavy Atom for Determining Phases in Sperm Whale Metmyoglobin

Xenon gas can be used as a heavy atom for determining phases in a protein. We demonstrate that an interpretable electron density map can be obtained for sperm whale metmyoglobin from a single xenon derivative using iterative single isomorphous replacement with the anomalous scattering method.

Bibliographic Details
Main Authors: Vitali, Jacqueline, Robbins, Arthur H., Almo, Steven C., Tilton, Robert F.
Format: Text
Language:unknown
Published: EngagedScholarship@CSU 1991
Subjects:
Online Access:https://engagedscholarship.csuohio.edu/sciphysics_facpub/183
https://engagedscholarship.csuohio.edu/cgi/viewcontent.cgi?article=1180&context=sciphysics_facpub
Description
Summary:Xenon gas can be used as a heavy atom for determining phases in a protein. We demonstrate that an interpretable electron density map can be obtained for sperm whale metmyoglobin from a single xenon derivative using iterative single isomorphous replacement with the anomalous scattering method.