Research Articles Genotoxicity of Field-Collected Inter-tidal Sediments From Cork Harbor, Ireland, to Juvenile Turbot (Scophthalmus maximus L.) as Measured by the Comet Assay
The alkaline single cell gel electrophoresis (SCGE) or Comet assay was employed to test the potential of surficial sediment collected from Cork Harbor, Ireland, to induce DNA damage in turbot (Scoph-thalmus maximus L.) in a laboratory exposure ex-periment. Turbot were exposed for 21 days to field-co...
| Main Authors: | , , , , |
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| Format: | Text |
| Language: | English |
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| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.648.9663 http://markhartl.sls.hw.ac.uk/Kilemade et al (2004).pdf |
| Summary: | The alkaline single cell gel electrophoresis (SCGE) or Comet assay was employed to test the potential of surficial sediment collected from Cork Harbor, Ireland, to induce DNA damage in turbot (Scoph-thalmus maximus L.) in a laboratory exposure ex-periment. Turbot were exposed for 21 days to field-collected sediment from Cork Harbor and from a relatively clean reference site at Ballyma-coda and sampled at 0, 7, 14, and 21 days. As a positive control for the sediment exposure experi-ment, a subsample of the turbot was exposed to cadmium chloride-spiked seawater. DNA damage analysis was performed on epidermal, gill, spleen, liver, and whole blood cell preparations. Liver, gill, and blood were the most sensitive tissues while a |
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