EXTENDED ABSTRACT ONLY – DO NOT CITE
A current problem for aquaculture of salmonid fish in Atlantic Canada is the susceptibility of these fish to cold water temperatures and ice. The development of freeze resistant salmon, therefore, would extend the range of salmon sea-pen aquaculture in Atlantic Canada. Previous attempts at producing...
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| Format: | Text |
| Language: | English |
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| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.631.5011 http://www-heb.pac.dfo-mpo.gc.ca/congress/2002/Performance/Hobbs.pdf |
| Summary: | A current problem for aquaculture of salmonid fish in Atlantic Canada is the susceptibility of these fish to cold water temperatures and ice. The development of freeze resistant salmon, therefore, would extend the range of salmon sea-pen aquaculture in Atlantic Canada. Previous attempts at producing freeze resistant transgenic salmon by introducing the type I antifreeze protein (AFP) gene from winter flounder have yielded much optimism (Fletcher et al., 1988). The AFP activity in the resulting transgenic salmon, however, was too low to confer freeze resistance to the fish (Hew et al., 1992). One possible reason for this may be due to the lack of an enzyme in salmon necessary to cleave the proAFP form of the type I AFP to produce the fully active mature protein. As an alternative, the gene encoding a type III AFP from ocean pout is being examined for the production of freeze resistance in salmon. The ocean pout AFP lacks the pro-sequence and is therefore fully active post-translationally and is constantly expressed in a variety of tissues (Fletcher et al., 1992). The ocean pout AFP gene was previously introduced into Atlantic salmon and shown to demonstrate Mendelian inheritance. |
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