The enzymatic production of biodiesel by methanolysis of cottonseed oil was studied using immobilized Candida antarctica lipase as catalyst in t-butanol solvent. Methyl ester production and triacylglycerol disappearance were followed by HPLC chromatography. It was found, using a batch system, that e...
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| Format: | Text |
| Language: | English |
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| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.625.8584 http://www.sinetinfo.com/pdf/cotton webpage/biodiesel from cotto seed.pdf |
| Summary: | The enzymatic production of biodiesel by methanolysis of cottonseed oil was studied using immobilized Candida antarctica lipase as catalyst in t-butanol solvent. Methyl ester production and triacylglycerol disappearance were followed by HPLC chromatography. It was found, using a batch system, that enzyme inhibition caused by undissolved methanol was eliminated by adding t-butanol to the reaction medium, which also gave a noticeable increase of reaction rate and ester yield. The eVect of t-butanol, methanol concentration and tem-perature on this system was determined. A methanolysis yield of 97 % was observed after 24 h at 50 °C with a reaction mixture containing 32.5 % t-butanol, 13.5 % methanol, 54 % oil and 0.017 g enzyme (g oil)¡1. With the same mixture, a 95 % ester yield was obtained using a one step Wxed bed continuous reactor with a Xow rate of 9.6 ml h¡1 (g enzyme)¡1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields. |
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