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The microcystin content and cyanobacterial community structure of Antarctic microbial mat samples collected from 40 ponds, lakes and hydro-terrestrial environments were investigated. Samples were collected from Bratina Island and four of the Dry Valleys; Wrights, Victoria, Miers and Marshall. Enzyme...

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Bibliographic Details
Main Authors: Susanna A. Wood, Doug Mountfort, Andrew I. Selwood, Patrick T. Holl, Jonathan Puddick, S. Craig Cary
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Subjects:
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.616.2143
http://researchcommons.waikato.ac.nz/bitstream/handle/10289/2830/Wood et al AEM final.pdf;jsessionid=DCE482392599513AC7BC844346A63781?sequence=1
Description
Summary:The microcystin content and cyanobacterial community structure of Antarctic microbial mat samples collected from 40 ponds, lakes and hydro-terrestrial environments were investigated. Samples were collected from Bratina Island and four of the Dry Valleys; Wrights, Victoria, Miers and Marshall. Enzyme-linked immunosorbent assays (ELISA), liquid chromatography mass spectrometry (LC-MS), and protein phosphatase inhibition assays (PP-2A) resulted in the identification of low levels (1- 16 mg/kg dry weight) of microcystins in all samples. A plot of indicative potencies of microcystins (ratio PP-2A:ELISA) versus total microcystins (ELISA) showed a general decrease in potency as total microcystin levels increased and a clustering of values from discrete geographic locations. LC-MS/MS analysis on selected samples identified eight novel microcystin congeners. The low energy collisional activation spectra were consistent with variants of [D-Asp 3] MC-RR