Brief report Isolation and cross-species amplification of microsatellite loci in the Siberian jay (Perisoreus infaustus)

• Main Authors: Bo-go Ran Lillandt, Staffan Bensch, Bengt Hansson, Liv Wennerberg, Torbjo Rn, Von Schantz
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Published: 2002
• Subjects: Helle; taiga
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.612.1290

Microsatellites are superior compared to other geneti-cal markers for parentage determination, because they can be analysed from tiny and partially degraded DNA-samples extracted from e.g. hairs or bird feath-ers (ELLEGREN 1992). However, bird genomes con-tain relatively few microsatellite loci (LONGMIRE et al. 1999; PRIMMER et al. 1997b). It is therefore a tedious process to isolate a set of markers that is sufficient for conclusive parentage analyses. Here we report on nine microsatellite markers that are polymorphic in the Siberian jay (Perisoreus infaustus), a resident family dwelling species occurring throughout the Eurasian taiga (HELLE and LILLANDT 1997). The markers were found using two methods; (1) isolating new mi-crosatellite sequences from a size-selected Siberian jay