Brief report Isolation and cross-species amplification of microsatellite loci in the Siberian jay (Perisoreus infaustus)
Microsatellites are superior compared to other geneti-cal markers for parentage determination, because they can be analysed from tiny and partially degraded DNA-samples extracted from e.g. hairs or bird feath-ers (ELLEGREN 1992). However, bird genomes con-tain relatively few microsatellite loci (LON...
Main Authors: | , , , , , |
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Other Authors: | |
Format: | Text |
Language: | English |
Published: |
2002
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Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.612.1290 |
Summary: | Microsatellites are superior compared to other geneti-cal markers for parentage determination, because they can be analysed from tiny and partially degraded DNA-samples extracted from e.g. hairs or bird feath-ers (ELLEGREN 1992). However, bird genomes con-tain relatively few microsatellite loci (LONGMIRE et al. 1999; PRIMMER et al. 1997b). It is therefore a tedious process to isolate a set of markers that is sufficient for conclusive parentage analyses. Here we report on nine microsatellite markers that are polymorphic in the Siberian jay (Perisoreus infaustus), a resident family dwelling species occurring throughout the Eurasian taiga (HELLE and LILLANDT 1997). The markers were found using two methods; (1) isolating new mi-crosatellite sequences from a size-selected Siberian jay |
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