DLA-DRB1, DQA1, and DQB1 Alleles and Haplotypes in North American Gray Wolves

• Main Authors: Lorna J. Kennedy, John M. Angles, Annette Barnes, Lindsey E. Carmichael, Alan D. Radford, William E. R. Ollier, George, M. Happ
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Canis lupus
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.589.7487; http://jhered.oxfordjournals.org/content/early/2007/07/19/jhered.esm051.full.pdf

The canine major histocompatibility complex contains highly polymorphic genes, many of which are critical in regulating immune response. Since domestic dogs evolved from Gray Wolves (Canis lupus), common DLA class II alleles should exist. Sequencing was used to characterize 175 Gray Wolves for DLA class II alleles, and data from 1856 dogs, covering 85 different breeds of mostly European origin, were available for comparison. Within wolves, 28 new alleles were identified, all occurring in at least 2 individuals. Three DLA-DRB1, 8 DLA-DQA1, and 6 DLA-DQB1 alleles also identified in dogs were present. Twenty-eight haplotypes were identified, of which 2 three-locus haplotypes, and many DLA-DQA1/DQB1 haplotypes, are also found in dogs. The wolves studied had relatively few dog DLA alleles and may therefore represent a remnant population descended from Asian wolves. The single European wolf included carried a haplotype found in both these North American wolves and in many dog breeds. Furthermore, one wolf DQB1 allele has been found in Shih Tzu, a breed of Asian origin. These data suggest that the wolf ancestors of Asian and European dogs may have had different gene pools, currently reflected in the DLA alleles present in dog breeds. The major histocompatibility complex (MHC) is essential for the presentation of foreign peptides to the vertebrate im-mune system (Brodsky et al. 1996; Weenink and Gautam