S1Supplemental Data Keratinocyte Differentiation Is Regulated by the Rho and ROCK Signaling Pathway

Supplemental Experimental Procedures permeabilized with PBS containing 0.2 % (v/v) Triton X100 (Sigma). After permeabilization, coverslips were incubated with primary and Reagents and Antibodies secondary antibodies as described previously [S4]. Entry of cells Antibodies used were as follows: Involu...

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Bibliographic Details
Main Authors: Rachel Mcmullan, Vicki H. Robertson, David J. Radford, Simon Broad, Fiona M. Watt, Alison Rowles, Daniel R. Croft, Michael F. Olson, Neil A. Hotchin
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Subjects:
Rho
Triton
Orca
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.575.7684
http://download.cell.com/current-biology/mmcs/journals/0960-9822/piis0960982203009060.mmc1.pdf
Description
Summary:Supplemental Experimental Procedures permeabilized with PBS containing 0.2 % (v/v) Triton X100 (Sigma). After permeabilization, coverslips were incubated with primary and Reagents and Antibodies secondary antibodies as described previously [S4]. Entry of cells Antibodies used were as follows: Involucrin (SY-5; Abcam, Cam- into S phase was analyzed via BrDU incorporation, for which a bridge, UK); Transglutaminase-1 (BC-1; gift of Dr R.H. Rice, Univer- commercially available kit (Boehringer Mannheim, Germany) was sity of California); Desmocollins 1 and 2 (Progen, Heidelberg, Ger- used. Immunostained cells were visualized with a Leica DMRB mi-many); phospho-MYPT1 (Thr696) (Upstate Biotechnology, Lake croscope equipped with a Hamamatsu ORCA camera, and images

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