RESPIRATION AND OXIDATIVE PHOSPHORYLATION O F MITOCHONDRIA FROM TISSUES AND ORGANS O F ANTARCTIC FISH*

• Main Authors: Mateus Sugizaki, Pedro Hklio Lucchiari, Maria Ivette, Carboni Malucelli, Av Nossa, Senhora Aparecida
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Antarctic; The Antarctic; Antarc*
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.566.2820; http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1997-Sugizaki.pdf

Abstract: Research has been carried out on the effect of temperature on the physiological properties of mitochondria isolated from liver, cardiac muscle and encephalon of the Antarctic fish Notothenia neglecta. Mitochondria were obtained in a medium containing 0.25 M mannitol, 0.075 M sucrose, l o 3 M EDTA, and l o 2 M TRIS, in l o 3 M phosphate buffer, pH 7.4. All assays were carried out by means of a polarograph assembled with an oxygen electrode possessing a temperature sensor. Respiration rates, oxidative phosphorylation and respiratory control values were assayed in regard to the effect of temperature. All preparations of liver, cardiac muscle and encephalon mitochondria displayed a very efficient respiration mecha-nism at 1°C No general pattern for the influence of temperature increase has been found in those preparations. On the other hand, encephalon mitochondria displayed an efficient system for ATP generation by oxidative phosphorylation, but sensitive to temperature increase. 1.