RESPIRATION AND OXIDATIVE PHOSPHORYLATION O F MITOCHONDRIA FROM TISSUES AND ORGANS O F ANTARCTIC FISH*

Abstract: Research has been carried out on the effect of temperature on the physiological properties of mitochondria isolated from liver, cardiac muscle and encephalon of the Antarctic fish Notothenia neglecta. Mitochondria were obtained in a medium containing 0.25 M mannitol, 0.075 M sucrose, l o 3...

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Bibliographic Details
Main Authors: Mateus Sugizaki, Pedro Hklio Lucchiari, Maria Ivette, Carboni Malucelli, Av Nossa, Senhora Aparecida
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Subjects:
Antarctic
The Antarctic
Antarc*
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.566.2820
http://polaris.nipr.ac.jp/~penguin/polarbiosci/issues/pdf/1997-Sugizaki.pdf
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Summary:Abstract: Research has been carried out on the effect of temperature on the physiological properties of mitochondria isolated from liver, cardiac muscle and encephalon of the Antarctic fish Notothenia neglecta. Mitochondria were obtained in a medium containing 0.25 M mannitol, 0.075 M sucrose, l o 3 M EDTA, and l o 2 M TRIS, in l o 3 M phosphate buffer, pH 7.4. All assays were carried out by means of a polarograph assembled with an oxygen electrode possessing a temperature sensor. Respiration rates, oxidative phosphorylation and respiratory control values were assayed in regard to the effect of temperature. All preparations of liver, cardiac muscle and encephalon mitochondria displayed a very efficient respiration mecha-nism at 1°C No general pattern for the influence of temperature increase has been found in those preparations. On the other hand, encephalon mitochondria displayed an efficient system for ATP generation by oxidative phosphorylation, but sensitive to temperature increase. 1.

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