Multiplication and haemadsorbing activity of infectious salmon anaemia virus in the established Atlantic salmon cell
Infectious salmon anaemia virus (ISAV), which pre-viously had never been isolated in any of the commercially available established fish cell lines, was successfully propagated in the continuous cell line Atlantic salmon (AS). The yield of infectious ISAV increased with the incubation time of virus-i...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Text |
| Language: | English |
| Published: |
1997
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| Subjects: | |
| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.555.5637 http://vir.sgmjournals.org/content/78/8/1891.full.pdf |
| Summary: | Infectious salmon anaemia virus (ISAV), which pre-viously had never been isolated in any of the commercially available established fish cell lines, was successfully propagated in the continuous cell line Atlantic salmon (AS). The yield of infectious ISAV increased with the incubation time of virus-inoculated cells, demonstrated by in vivo infectivity trials in groups of Atlantic salmon. Trypsin treat-ment of the virus was not necessary for primary infection of AS cells with salmon-grown ISAV. The infection was non-cytopathic, but it was possible to detect virus-infected cells by a haemadsorption centre assay using Atlantic salmon erythrocytes. Pleomorphic enveloped virus particles were seen by transmission electron microscopy of infected AS |
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