Heterogeneity in the genome RNAs and polypeptides of five members of a novel group of rotavirus-like viruses isolated from aquatic animals
Biochemical characteristics of five rotavirus-like vir-uses isolated from striped bass (Morone saxatilis), turbot (Scophthalmus maximus), smelt (Osmerus mor-dax) and Atlantic salmon (Salmo salar) in North America and Europe were compared. The genome of each isolate was composed of 11 segments of dsR...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Text |
| Language: | English |
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| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.546.2777 http://vir.sgmjournals.org/content/72/1/181.full.pdf |
| Summary: | Biochemical characteristics of five rotavirus-like vir-uses isolated from striped bass (Morone saxatilis), turbot (Scophthalmus maximus), smelt (Osmerus mor-dax) and Atlantic salmon (Salmo salar) in North America and Europe were compared. The genome of each isolate was composed of 11 segments of dsRNA and each isolate had a unique electropherotype in polyacrylamide gels. Agarose gel electrophoresis showed similar RNA profiles for all four isolates from North America, whereas the RNA profile of the isolate from Europe was different. Analysis of virion proteins revealed that each virus had five structural proteins ranging in Mr from 130 000 to 34 000. Each isolate had a unique polypeptide profile but their overall polypep-tide patterns were similar. Reciprocal RNA-RNA blot hybridization demonstrated that all these rotavirus-like viruses cross-hybridized with each other except for the isolate from Europe which did not hybridize with the RNA from any of the other isolates. No genetic relationship was found between these rotavirus-like viruses of fish and a true group A rotavirus (SA11). Viruses belonging to the family Reoviridae have been isolated from different species of fish and shellfish |
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