HOS9 Mediated Cold Acclimation Outside the CBF Regulon
The genetic basis for plant cold tolerance, that is, which genes are needed to convey cold tolerance to plants, has been a long standing goal of plant scientists. Two general molecular genetic approaches toward achieving this goal have been implemented in the past decade or two using the model plant...
| Main Authors: | , , |
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| Format: | Text |
| Language: | English |
| Published: |
2004
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| Subjects: | |
| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.540.9681 http://www.isb.vt.edu/news/2004/artspdf/oct0405.pdf |
| Summary: | The genetic basis for plant cold tolerance, that is, which genes are needed to convey cold tolerance to plants, has been a long standing goal of plant scientists. Two general molecular genetic approaches toward achieving this goal have been implemented in the past decade or two using the model plant Arabidopsis. First, directly screening for plants with altered freezing tolerance has yielded several mutants such as the sfr mutants which are sensitive to freezing1 and the eskimo mu-tant2 that displays increased freezing tolerance. So far, few of the genes responsible for these altered freezing phenotypes have been identified. Only recently has one of the SFR mutant genes, sfr2, been cloned3. Another approach utilizing the identification of genes that have altered expression after exposure to cold temperatures such as the kin (cold induced), lti (low temperature induced) and cor (cold regulated) genes has resulted in the identification and cloning of more genes that are required for cold tolerance. After the identification of the cold/desiccation induction recognition sequence motifs called CRT/DRE (C repeat/dehy-dration responsive element) in the promoters of cor target gene4, Stockinger et al.5 identified the CBF1 (cor binding fac-tor) gene that encodes an APETELA2/ethylene response element class transcription factor that regulates the expression of cor target genes by binding to the CRT/DRE promoter region. Later, using yeast one-hybrid screening, other members of the CBF transcription factor family were identified and called DRE binding (DREB) proteins6. Overexpression of a number of the CBF transcription factor genes in Arabidopsis has resulted in increased stress toler- |
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