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A Gram-negative, strictly aerobic, short rod-shaped, non-motile bacterial strain designated 4M24T was isolated from cotton-waste compost. Analysis of the 16S rRNAgene sequence of strain 4M24T revealed that it is a member of the genus Sphingobacterium, sharing 88.5–94.5 % sequence similarity with typ...

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Bibliographic Details
Main Authors: Soon-wo Kwon, Seung-joo Go, Erko Stackebr, Hang-yeon Weon
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
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Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.534.9471
http://ijsb.sgmjournals.org/content/57/7/1590.full.pdf
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Summary:A Gram-negative, strictly aerobic, short rod-shaped, non-motile bacterial strain designated 4M24T was isolated from cotton-waste compost. Analysis of the 16S rRNAgene sequence of strain 4M24T revealed that it is a member of the genus Sphingobacterium, sharing 88.5–94.5 % sequence similarity with type strains of the genus Sphingobacterium and being most closely related to Sphingobacterium daejeonense TR6-04T (94.5 % sequence similarity) and Sphingobacterium mizutaii ATCC 33299T (92.2 % similarity). The major fatty acids of strain 4M24T grown on trypticase soy agar medium were summed feature 3 (iso-C15: 0 2-OH and/or C16: 1v7c; 37.5%), iso-C15: 0 (29.5%) and iso-C17: 0 3-OH (19.7%). The G+C content of the genomic DNA was 42.3 mol%. On the basis of phenotypic and genotypic characteristics, strain 4M24T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium composti sp. nov. is proposed. The type strain is 4M24T (=KACC 11313T=DSM 18850T). Members of the genus Sphingobacterium Yabuuchi et al. 1983 have been isolated from Antarctic soil, clinical speci-mens (blood, urine and the uteruses of human patients) and compost composed of cow dung and rice straw (Holmes