Microsatellite markers for the common vole (Microtus arvalis) and their cross-species utility

Abstract Fragmentation of natural habitats of the com-mon vole (Microtus arvalis) provides an excellent model system to study the consequences of restricted gene flow and small population sizes for isolated populations. Here we describe the isolation and characteristics of 10 autoso-mal and one X-li...

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Bibliographic Details
Main Authors: Barbara Walser, Æ Gerald Heckel
Other Authors: The Pennsylvania State University CiteSeerX Archives
Format: Text
Language:English
Published: 2007
Subjects:
Online Access:http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.534.3929
http://www.unifr.ch/biol/ecology/haag/group/barbara_walser/Walser%26Heckel.pdf
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Summary:Abstract Fragmentation of natural habitats of the com-mon vole (Microtus arvalis) provides an excellent model system to study the consequences of restricted gene flow and small population sizes for isolated populations. Here we describe the isolation and characteristics of 10 autoso-mal and one X-linked microsatellite marker. These new markers were tested in 24 voles from a natural population in eastern Germany. Loci were highly polymorphic with numbers of alleles per locus ranging from three to 26 and expected heterozygosities from 0.51 to 0.97. All loci except for the X-linked locus Mar105 followed Hardy–Weinberg expectations. Cross-species amplifications revealed that most loci were polymorphic as well in M. agrestis, M. thomasi, and M. pennsylvanicus.