.Aquaculture 173 1999 347–357 Aneuploid Pacific oyster Crassostrea gigas /Thunberg as incidentals from triploid production
Triploids have become an important part of the oyster aquaculture industry. Triploids can be . .produced by two methods: 1 inhibiting polar body II in normal zygotes; and 2 mating diploids and tetraploids. Both methods can theoretically generate some incidental aneuploids, which in higher animals ar...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Text |
| Language: | English |
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1998
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| Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.530.9392 http://www.ccb.ufsc.br/~andre/artigos/Wang99.pdf |
| Summary: | Triploids have become an important part of the oyster aquaculture industry. Triploids can be . .produced by two methods: 1 inhibiting polar body II in normal zygotes; and 2 mating diploids and tetraploids. Both methods can theoretically generate some incidental aneuploids, which in higher animals are often lethal or associated with growth retardation. In this study, we investigated .the occurrence of aneuploids in three replicates of a normal diploid group 2N, a cytochalasin B .induced triploid 3nCB and a mated triploid group from diploid female = tetraploid male mating .3nDT, and documented their growth performance. No aneuploids were found in normal diploid groups, and all diploid oysters had exactly the expected diploid number y20 chromosomes. Two . .of the 90 oysters 2.2 % from 3nCB groups were aneuploids: one with 19 chromosomes 2ny1 . .and one with 29 chromosomes 3ny1. In 3nDT groups, 18 of the 90 oysters 20 % were .aneuploids, which included two oysters with 28 chromosomes 3ny2, 10 with 29 chromosomes . .3ny1 and six with 31 chromosomes 3nq1. The large numbers of aneuploids from 3nDT groups suggested that meiosis in paternal tetraploids was prone to segregation error. Triploids from 3nDT groups were significantly bigger than normal diploids and 3nCB triploids. Aneuploids were not significantly different from 3nDT triploids in body size. But like 3nDT triploids, aneuploids as a group were significantly bigger than normal diploids and 3nCB triploids. Results |
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