DOI 10.1007/s00203-006-0198-1ORIGINAL PAPER Characterization of Francisella sp., GM2212, the Wrst Francisella isolate from marine Wsh, Atlantic cod
ously isolated from diseased farmed Atlantic cod Gadus morhua in Norway is characterized. The com-plete 16S rDNA, 16S–23S intergenic spacer, 23S rDNA, 23S–5S intergenic spacer, 5S rDNA, FopA, lipoprotein TUL4 (LpnA), malate dehydrogenase and a hypothetical lipoprotein (LpnB) is sequenced and compare...
Main Authors: | , , , , , |
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Format: | Text |
Language: | English |
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Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.516.2129 http://www.torsk.net/fileadmin/frisk_torsk/Fulltekst.pdf |
Summary: | ously isolated from diseased farmed Atlantic cod Gadus morhua in Norway is characterized. The com-plete 16S rDNA, 16S–23S intergenic spacer, 23S rDNA, 23S–5S intergenic spacer, 5S rDNA, FopA, lipoprotein TUL4 (LpnA), malate dehydrogenase and a hypothetical lipoprotein (LpnB) is sequenced and compared with Francisella tularensis and Francisella philomiragia. All these sequences support a close rela-tionship between GM2212T and F. philomiragia. The bacterium grows at 10–25°C with an optimum at about 20°C, a temperature range clearly diVerent from F. tularensis and F. philomiragia. GM2212T is catalase-positive, indole positive, oxidase-negative, do not produce H2S in Triple Sugar Iron agar, and does not hydrolyze gelatin, is resistant to erythromycin and sus-ceptible to ceftazidime, the latter Wve characteristics separating it from F. philomiragia. Cysteine enhances growth. Acid is produced from D-glucose, maltose, sucrose (weak) but not from lactose or glycerol. GM2212T grows on both MacConkey agar and in nutrient broth (6 % NaCl). The bacterium is resistant to trimethoprim-sulfamethoxazole, penicillines, cefur-oxime and erythromycin; but is susceptible to ceftazi-dime, tetracycline, gentamicin, ciproXoxacin. Based on the molecular and phenotypical characteristics, we sug-gest that this GM2212 isolate, may represent a new species of Francisella. Isolate GM2212T (=CNCM I-3481T = CNCM I-3511T = DSM 18777T). |
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